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Rim aperture of yeast autophagic membranes balances cargo inclusion with vesicle maturation.
Shatz, Oren; Fraiberg, Milana; Isola, Damilola; Das, Shubhankar; Gogoi, Olee; Polyansky, Alexandra; Shimoni, Eyal; Dadosh, Tali; Dezorella, Nili; Wolf, Sharon G; Elazar, Zvulun.
Afiliación
  • Shatz O; Departments of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Fraiberg M; Departments of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Isola D; Departments of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Das S; Departments of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Gogoi O; Departments of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Polyansky A; Departments of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Shimoni E; Chemical Research Support, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Dadosh T; Chemical Research Support, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Dezorella N; Chemical Research Support, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Wolf SG; Chemical Research Support, The Weizmann Institute of Science, 76100 Rehovot, Israel.
  • Elazar Z; Departments of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel. Electronic address: zvulun.elazar@weizmann.ac.il.
Dev Cell ; 59(7): 911-923.e4, 2024 Apr 08.
Article en En | MEDLINE | ID: mdl-38447569
ABSTRACT
Autophagy eliminates cytoplasmic material by engulfment in membranous vesicles targeted for lysosome degradation. Nonselective autophagy coordinates sequestration of bulk cargo with the growth of the isolation membrane (IM) in a yet-unknown manner. Here, we show that in the budding yeast Saccharomyces cerevisiae, IMs expand while maintaining a rim sufficiently wide for sequestration of large cargo but tight enough to mature in due time. An obligate complex of Atg24/Snx4 with Atg20 or Snx41 assembles locally at the rim in a spatially extended manner that specifically depends on autophagic PI(3)P. This assembly stabilizes the open rim to promote autophagic sequestration of large cargo in correlation with vesicle expansion. Moreover, constriction of the rim by the PI(3)P-dependent Atg2-Atg18 complex and clearance of PI(3)P by Ymr1 antagonize rim opening to promote autophagic maturation and consumption of small cargo. Tight regulation of membrane rim aperture by PI(3)P thus couples the mechanism and physiology of nonselective autophagy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos