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Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression.
Song, Pengkang; Zhao, Jiamin; Zhang, Weipeng; Li, Xuying; Ji, Bingzhen; Zhao, Junxing.
Afiliación
  • Song P; College of Animal Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, P. R. China.
  • Zhao J; Shanxi Key Laboratory of Animal Genetics Resource Utilization and Breeding, Taigu, P. R. China.
  • Zhang W; College of Animal Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, P. R. China.
  • Li X; Shanxi Key Laboratory of Animal Genetics Resource Utilization and Breeding, Taigu, P. R. China.
  • Ji B; College of Animal Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, P. R. China.
  • Zhao J; College of Animal Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, P. R. China.
BMC Genomics ; 25(1): 244, 2024 Mar 05.
Article en En | MEDLINE | ID: mdl-38443816
ABSTRACT

BACKGROUND:

Vitamin A and retinoic acid (RA, a metabolite of vitamin A), are inextricably involved to the development of skeletal muscle in animals. However, the mechanisms regulating skeletal muscle development by vitamin A remain poorly reported. The current study designed to investigate the underlying mechanism of vitamin A affecting myogenic differentiation of lamb myoblasts through transcriptome sequencing (RNA-Seq) and gene function validation experiments. It provides a theoretical basis for elucidating the regulation of vitamin A on skeletal muscle development as well as for improving the economic benefits of the mutton sheep industry.

RESULTS:

Newborn lambs were injected with 7,500 IU vitamin A, and longissimus dorsi (LD) muscle tissue was surgically sampled for RNA-Seq analysis and primary myoblasts isolation at 3 weeks of age. The results showed that a total of 14 down-regulated and 3 up-regulated genes, were identified between control and vitamin A groups. Among them, BHLHE40 expression was upregulated in vitamin A group lambs. Furthermore, BHLHE40 expression is significantly increased after initiation of differentiation in myoblasts, and RA addition during differentiation greatly promoted BHLHE40 mRNA expression. In vitro, RA inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation through BHLHE40. Moreover, BHLHE40 was proved to inhibit the expression of the DNA binding inhibitor 3 (ID3), and meanwhile, ID3 could effectively promote myoblasts proliferation and inhibit myoblasts myogenic differentiation.

CONCLUSIONS:

Taken together, our results suggested that vitamin A inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation by inhibiting ID3 expression through BHLHE40.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tretinoina / Vitamina A Límite: Animals Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tretinoina / Vitamina A Límite: Animals Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido