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Detecting and exploring kidney-derived extracellular vesicles in plasma.
Komatsu, Shintaro; Kato, Noritoshi; Kitai, Hiroki; Funahashi, Yoshio; Noda, Yuhei; Tsubota, Shoma; Tanaka, Akihito; Sato, Yuka; Maeda, Kayaho; Saito, Shoji; Furuhashi, Kazuhiro; Ishimoto, Takuji; Kosugi, Tomoki; Maruyama, Shoichi; Kadomatsu, Kenji.
Afiliación
  • Komatsu S; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Kato N; Division of Molecular Oncology, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Kitai H; Department of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Funahashi Y; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan. n-kato@med.nagoya-u.ac.jp.
  • Noda Y; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Tsubota S; Department of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Tanaka A; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Sato Y; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Maeda K; Department of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Saito S; Department of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Furuhashi K; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Ishimoto T; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Kosugi T; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Maruyama S; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
  • Kadomatsu K; Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
Clin Exp Nephrol ; 28(7): 617-628, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38436899
ABSTRACT

BACKGROUND:

Extracellular vesicles (EVs) have received considerable attention as ideal biomarkers for kidney diseases. Most reports have focused on urinary EVs, that are mainly derived from the cells in the urinary tract. However, the detection and the application of kidney-derived EVs in plasma remains uncertain.

METHODS:

We examined the kidney-derived small EVs (sEVs) in plasma that were supposedly released from renal mesangial and glomerular endothelial cells, using clinical samples from healthy controls and patients with kidney transplants. Plasma from healthy controls underwent ultracentrifugation, followed by on-bead flow cytometry, targeting α8 integrin, an antigen-specific to mesangial cells. To confirm the presence of kidney-derived sEVs in peripheral blood, plasma from ABO-incompatible kidney transplant recipients was ultracentrifuged, followed by western blotting for donor blood type antigens.

RESULTS:

Immunohistochemistry and immunoelectron microscopy confirmed α8 integrin expression in kidney mesangial cells and their sEVs. The CD9-α8 integrin double-positive sEVs were successfully detected using on-bead flow cytometry. Western blot analysis further revealed transplanted kidney-derived sEVs containing blood type B antigens in non-blood type B recipients, who had received kidneys from blood type B donors. Notably, a patient experiencing graft kidney loss exhibited diminished signals of sEVs containing donor blood type antigens.

CONCLUSION:

Our findings demonstrate the potential usefulness of kidney-derived sEVs in plasma in future research for kidney diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Riñón / Vesículas Extracelulares Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Clin Exp Nephrol Asunto de la revista: NEFROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Riñón / Vesículas Extracelulares Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Clin Exp Nephrol Asunto de la revista: NEFROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón