Development of surface-enhanced Raman scattering-sensing Method by combining novel Ag@Au core/shell nanoparticle-based SERS probe with hybridization chain reaction for high-sensitive detection of hepatitis C virus nucleic acid.

Peng, Ruiying; Qi, Wenchen; Deng, Ting; Si, Yanmei; Li, Jishan

Peng, Ruiying; Qi, Wenchen; Deng, Ting; Si, Yanmei; Li, Jishan.

Afiliación

Peng R; Institute of Applied Chemistry, School of Science, Central South University of Forestry and Technology, Changsha, 410004, People's Republic of China.
Qi W; State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People's Republic of China.
Deng T; State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People's Republic of China.
Si Y; Institute of Applied Chemistry, School of Science, Central South University of Forestry and Technology, Changsha, 410004, People's Republic of China. muzi_dt@hotmail.com.
Li J; Institute of Forensic Medicine and Laboratory Medicine, Jining Medical University, Jining, 272067, People's Republic of China.

Anal Bioanal Chem ; 416(10): 2515-2525, 2024 Apr.

Article en En | MEDLINE | ID: mdl-38436691

ABSTRACT

ABSTRACT

The ultrasensitive detection of hepatitis C virus (HCV) nucleic acid is crucial for the early diagnosis of hepatitis C. In this study, by combining Ag@Au core/shell nanoparticle (Ag@AuNP)-based surface-enhanced Raman scattering (SERS) tag with hybridization chain reaction (HCR), a novel SERS-sensing method was developed for the ultrasensitive detection of HCV nucleic acid. This SERS-sensing system comprised two different SERS tags, which were constructed by modifying Ag@AuNP with a Raman reporter molecule of 4-ethynylbezaldehyde, two different hairpin-structured HCR sequences (H1 or H2), and a detection plate prepared by immobilizing a capture DNA sequence onto the Ag@AuNP layer surface of the detection wells. When the target nucleic acid was present, the two SERS tags were captured on the surface of the Ag@AuNP-coated detection well to generate many "hot spots" through HCR, forming a strong SERS signal and realizing the ultrasensitive detection of the target HCV nucleic acid. The limit of detection of the SERS-sensing method for HCV nucleic acid was 0.47 fM, and the linear range was from 1 to 105 fM.

Asunto(s)

Hepatitis C; Nanopartículas del Metal; Nanopartículas; Ácidos Nucleicos; Humanos; Hepacivirus/genética; Espectrometría Raman/métodos; Oro

Palabras clave

Ag@Au core/shell nanoparticle; Hepatitis C virus; Hybridization chain reaction; Surface-enhanced Raman scattering; Ultrasensitive detection

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Hepatitis C / Nanopartículas / Nanopartículas del Metal Límite: Humans Idioma: En Revista: Anal Bioanal Chem Año: 2024 Tipo del documento: Article Pais de publicación: Alemania

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