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Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat.
Lee, Da Young; Han, Dahee; Lee, Seung Yun; Yun, Seung Hyeon; Lee, Juhyun; Mariano, Ermie; Choi, Yeongwoo; Kim, Jin Soo; Park, Jinmo; Hur, Sun Jin.
Afiliación
  • Lee DY; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Han D; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Lee SY; Division of Animal Science, Division of Applied Life Science (BK21 Four), Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Yun SH; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Lee J; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Mariano E; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Choi Y; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Kim JS; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Park J; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • Hur SJ; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
Food Chem X ; 21: 101202, 2024 Mar 30.
Article en En | MEDLINE | ID: mdl-38434697
ABSTRACT
Fetal bovine serum (FBS) substitution remains one of the challenges to the realization of cultured meat production in the marketplace. In this study, three methods were developed to extract a substitute for FBS using egg white extract (EWE) using 25 mM CaCl2/2.5 % ammonium sulfate/citric acid (A); ethyl alcohol (B); and 5 % ammonium sulfate/citric acid (C). B EWE can effectively replace up to 50 % of FBS in growth media (10 % of the total). Ovalbumin in the extracts can promote cell proliferation, and components along the 12 kDa protein band have the potential to inhibit cell proliferation. Chick primary muscle cells applied with B EWE, an edible material that improved the cost and time efficiency of cultured meat production, effectively proliferated/differentiated. Therefore, EWE extracted using ethyl alcohol may be used as an FBS substitute to reduce animal sacrifices and should be considered a viable alternative to FBS for cultured meat.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Chem X Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Chem X Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos