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Pyrococcus furiosus argonaute based Alicyclobacillus acidoterrestrsis detection in fruit juice.
Shi, Yiheng; Tan, Zishan; Wu, Di; Wu, Yongning; Li, Guoliang.
Afiliación
  • Shi Y; School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • Tan Z; School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • Wu D; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, United Kingdom.
  • Wu Y; School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; NHC Key Laboratory of Food Safety Risk Assessment, Food Safety Research Unit (2019RU014) of Chinese Academy of Medical Science, China National Center for Food Safety Risk Assessment, Beijing 10
  • Li G; School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. Electronic address: 61254368@163.com.
Food Microbiol ; 120: 104475, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38431321
ABSTRACT
Alicyclobacillus acidoterrestris is the major threat to fruit juice for its off-odor producing characteristic. In this study, Pyrococcus furiosus Argonaute (PfAgo), a novel endonuclease with precise DNA cleavage activity, was used for A. acidoterrestrisdetection, termed as PAD. The partially amplified 16 S rRNA gene of A. acidoterrestris can be cleaved by PfAgo activated by a short 5'-phosphorylated single strand DNA, producing a new guide DNA (gDNA). Then, PfAgo was activated by the new gDNA to cut a molecular beacon (MB) with fluorophore-quencher reporter, resulting in the recovery of fluorescence. The fluorescent intensity is positively related with the concentration of A. acidoterrestris. The PAD assay showed excellent specificity and sensitivity as low as 101 CFU/mL, which can be a powerful tool for on-site detection of A. acidoterrestris in fruit juice industry in the future, reducing the economic loss.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pyrococcus furiosus / Alicyclobacillus Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pyrococcus furiosus / Alicyclobacillus Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido