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Optimization of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for the quantitative determination of 3,4-methylenedioxy-methamphetamine (MDMA) and its phase-1 metabolites in human biological fluids.
Sprega, Giorgia; Kobidze, Giorgi; Lo Faro, Alfredo Fabrizio; Pichini, Simona; Farkas, Tivadar; Tini, Anastasio; Mskhiladze, Antonina; Busardò, Francesco Paolo; Chankvetadze, Bezhan.
Afiliación
  • Sprega G; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona 60121, Italy.
  • Kobidze G; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona 60121, Italy.
  • Lo Faro AF; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona 60121, Italy. Electronic address: a.lofaro@staff.univpm.it.
  • Pichini S; National Centre on Addiction and Doping, Istituto Superiore di Sanità, Rome, Italy.
  • Farkas T; Phenomenex Inc., 411 Madrid Ave, Torrance, CA 90501, USA.
  • Tini A; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona 60121, Italy.
  • Mskhiladze A; Faculty of Natural Sciences, Mathematics, Technologies and Pharmacy, Sokhumi State University, Polytkovskaya 61, Tbilisi 0186, Georgia.
  • Busardò FP; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona 60121, Italy.
  • Chankvetadze B; Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, I. Chavchavadze Ave 1, Tbilisi 0179, Georgia.
J Pharm Biomed Anal ; 243: 116076, 2024 Jun 15.
Article en En | MEDLINE | ID: mdl-38430614
ABSTRACT
Recently we published in this journal an enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitative determination of 3,4-methylenedioxymethamphetamine (MDMA) and its major phase-1 metabolites, 4-hydroxy-3-methoxyamphetamine (HMA), 4-hydroxy-3-methoxymethamphetamine (HMMA) and 3,4-methylenedioxyamphetamine (MDA) in human plasma, sweat, oral fluid and urine. Since we did not achieve simultaneous enantioseparation of all 4 compounds with a single chiral column, two amylose-based chiral columns were used alternatively. Further optimization of the mobile phase in the present study enabled baseline separation of all four pairs of enantiomers on a single Lux AMP column. In addition, by optimization of the column dimension and applied flow-rate it became possible to complete the separation within 6 minutes. These new methods were applied to the analysis of human plasma, oral fluid and urine. While results on the concentration of MDMA and its metabolites in various biological fluids were reported in our recent publication, in the present study an attempt was made to hydrolyze glucuronides in urine samples by using alternatively, hydrochloric acid or glucuronidase and to evaluate the effect of hydrolysis on the concentration and enantiomeric distribution of hydroxy metabolites of MDMA such as HMA and HMMA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: N-Metil-3,4-metilenodioxianfetamina / Lactatos / Metanfetamina / 3,4-Metilenodioxianfetamina Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: N-Metil-3,4-metilenodioxianfetamina / Lactatos / Metanfetamina / 3,4-Metilenodioxianfetamina Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido