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Transcriptomic and intervention evidence reveals domestic dogs as a promising model for anti-inflammatory investigation.
Zeng, Min; Zhou, Tong; Li, Zhiyu; Li, Guimei; Zhang, Shurun; Wang, Lu; Huang, Qing-Guo; Li, Ju-Dong; Samarawickrama, P Nadeeshika; He, Yonghan; Wang, Guo-Dong.
Afiliación
  • Zeng M; Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
  • Zhou T; Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China.
  • Li Z; Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
  • Li G; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
  • Zhang S; Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
  • Wang L; Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
  • Huang QG; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
  • Li JD; Kunming Police Dog Base of the Chinese Ministry of Public Security, Kunming, China.
  • Samarawickrama PN; Kunming Police Dog Base of the Chinese Ministry of Public Security, Kunming, China.
  • He Y; Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
  • Wang GD; Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China.
Aging Cell ; 23(5): e14127, 2024 05.
Article en En | MEDLINE | ID: mdl-38426629
ABSTRACT
Domestic dogs have great potential to expand our understanding of the determinants of aging. To understand the aging pattern of domestic dogs and evaluate whether they can be used as an aging model, we performed RNA sequencing on white blood cells from domestic dogs aged 1-9 years and treated aged dogs with classical antiaging approaches. We obtained 30 RNA sequencing libraries and identified 61 age-associated genes with dynamic changes, the majority of which were related to metabolism and immune function, which may be predominant biomarkers for aging in dogs. We next treated aged dogs with canine mesenchymal stem cells (cMSCs), nicotinamide mononucleotide, and rapamycin to determine whether and how they responded to the antiaging interventions. The results showed that these treatments can significantly reduce the level of inflammatory factors (IL-6 and TNF-α). MSCs effectively improved the heart functions of aged dogs. Three key potential age-related genes (PYCR1, CCRL2, and TOX) were reversed by MSC treatment, two of which (CCRL2 and TOX) are implicated in immunity. Overall, we profiled the transcriptomic pattern of domestic dogs and revealed that they may be a good model of aging, especially in anti-inflammatory investigations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcriptoma Límite: Animals Idioma: En Revista: Aging Cell Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcriptoma Límite: Animals Idioma: En Revista: Aging Cell Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido