Your browser doesn't support javascript.
loading
Application of bulk segregant RNA-Seq (BSR-Seq) and allele-specific primers to study soybean powdery mildew resistance.
Huang, Cheng-Chun; Lin, Chen-Hsiang; Lin, Yu-Cheng; Chang, Hao-Xun.
Afiliación
  • Huang CC; Master Program for Plant Medicine, National Taiwan University, Taipei, 106319, Taiwan.
  • Lin CH; Taoyuan District Agricultural Research and Extension Station. Ministry of Agriculture, Taoyuan, 327005, Taiwan.
  • Lin YC; Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, 106319, Taiwan.
  • Chang HX; Department of Ecology and Evolutionary Biology, The University of Michigan, Ann Arbor, MI, 48109, USA.
BMC Plant Biol ; 24(1): 155, 2024 Mar 01.
Article en En | MEDLINE | ID: mdl-38424508
ABSTRACT

BACKGROUND:

Powdery mildew (PM) is one of the important soybean diseases, and host resistance could practically contribute to soybean PM management. To date, only the Rmd locus on chromosome (Chr) 16 was identified through traditional QTL mapping and GWAS, and it remains unclear if the bulk segregant RNA-Seq (BSR-Seq) methodology is feasible to explore additional PM resistance that might exist in other varieties.

RESULTS:

BSR-Seq was applied to contrast genotypes and gene expressions between the resistant bulk (R bulk) and the susceptible bulk (S bulk), as well as the parents. The ∆(SNP-index) and G' value identified several QTL and significant SNPs/Indels on Chr06, Chr15, and Chr16. Differentially expressed genes (DEGs) located within these QTL were identified using HISAT2 and Kallisto, and allele-specific primers (AS-primers) were designed to validate the accuracy of phenotypic prediction. While the AS-primers on Chr06 or Chr15 cannot distinguish the resistant and susceptible phenotypes, AS-primers on Chr16 exhibited 82% accuracy prediction with an additive effect, similar to the SSR marker Satt431.

CONCLUSIONS:

Evaluation of additional AS-primers in the linkage disequilibrium (LD) block on Chr16 further confirmed the resistant locus, derived from the resistant parental variety 'Kaohsiung 11' ('KS11'), not only overlaps with the Rmd locus with unique up-regulated LRR genes (Glyma.16G213700 and Glyma.16G215100), but also harbors a down-regulated MLO gene (Glyma.16G145600). Accordingly, this study exemplified the feasibility of BSR-Seq in studying biotrophic disease resistance in soybean, and showed the genetic makeup of soybean variety 'KS11' comprising the Rmd locus and one MLO gene.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Resistencia a la Enfermedad Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Resistencia a la Enfermedad Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido