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Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol.
McCarthy, Clíona M; McKevitt, Kevin L; Connolly, Sinéad A; Andersson, Isabel; Leahy, Fiona C; Egan, Siobhan; Moloney, Michael A; Kavanagh, Eamon G; Peirce, Colin; Cunnane, Eoghan M; McGourty, Kieran D; Walsh, Michael T; Mulvihill, John J E.
Afiliación
  • McCarthy CM; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
  • McKevitt KL; School of Engineering, University of Limerick, Limerick, Ireland.
  • Connolly SA; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
  • Andersson I; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
  • Leahy FC; School of Engineering, University of Limerick, Limerick, Ireland.
  • Egan S; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
  • Moloney MA; School of Engineering, University of Limerick, Limerick, Ireland.
  • Kavanagh EG; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
  • Peirce C; Department of Colorectal Surgery, University Hospital Limerick, Limerick, Ireland.
  • Cunnane EM; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
  • McGourty KD; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
  • Walsh MT; Department of Colorectal Surgery, University Hospital Limerick, Limerick, Ireland.
  • Mulvihill JJE; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
PLoS One ; 19(2): e0297618, 2024.
Article en En | MEDLINE | ID: mdl-38422111
ABSTRACT
Microindentation of fresh biological tissues is necessary for the creation of 3D biomimetic models that accurately represent the native extracellular matrix microenvironment. However, tissue must first be precisely sectioned into slices. Challenges exist in the preparation of fresh tissue slices, as they can tear easily and must be processed rapidly in order to mitigate tissue degradation. In this study, we propose an optimised mounting condition for microindentation and demonstrate that embedding tissue in a mixture of 2.5% agarose and 1.5% gelatin is the most favourable method of tissue slice mounting for microindentation. This protocol allows for rapid processing of fresh biological tissue and is applicable to a variety of tissue types.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomimética / Matriz Extracelular Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomimética / Matriz Extracelular Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos