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Profiling of Long Non-Coding RNAs in Auricular Cartilage of Patients with Isolated Microtia.
Yang, Run; Fu, Yaoyao; Li, Chenlong; Chen, Yin; He, Aijuan; Jiang, Xin; Ma, Jing; Zhang, Tianyu.
Afiliación
  • Yang R; Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye & ENT Hospital of Fudan University, Shanghai, China.
  • Fu Y; Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye & ENT Hospital of Fudan University, Shanghai, China.
  • Li C; Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye & ENT Hospital of Fudan University, Shanghai, China.
  • Chen Y; Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye & ENT Hospital of Fudan University, Shanghai, China.
  • He A; Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye & ENT Hospital of Fudan University, Shanghai, China.
  • Jiang X; Medical Laboratory of Nantong Zhongke, Department of Bioinformatics, Nantong, Jiangsu, China.
  • Ma J; Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye & ENT Hospital of Fudan University, Shanghai, China.
  • Zhang T; Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye & ENT Hospital of Fudan University, Shanghai, China.
Genet Test Mol Biomarkers ; 28(2): 50-58, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38416666
ABSTRACT

Introduction:

Microtia is the second most common maxillofacial birth defect worldwide. However, the involvement of long non-coding RNAs (lncRNAs) in isolated microtia is not well understood. This study aimed at identifying lncRNAs that regulate the expression of genes associated with isolated microtia.

Methods:

We used our microarray data to analyze the expression pattern of lncRNA in the auricular cartilage tissues from 10 patients diagnosed with isolated microtia, alongside 15 control subjects. Five lncRNAs were chosen for validation using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Results:

We identified 4651 differentially expressed lncRNAs in the auricular cartilage from patients with isolated microtia. By Gene Ontology/Kyoto Encyclopedia of Genes and Genomes pathway (GO/KEGG) analysis, we identified 27 differentially expressed genes enriched in pathways associated with microtia. In addition, we predicted 9 differentially expressed genes as potential cis-acting targets of 12 differentially expressed lncRNAs. Our findings by qRT-PCR demonstrate significantly elevated expression levels of ZFAS1 and DAB1-AS1, whereas ADIRF-AS1, HOTAIRM1, and EPB41L4A-AS1 exhibited significantly reduced expression levels in the auricular cartilage tissues of patients with isolated microtia.

Conclusions:

Our study sheds light on the potential involvement of lncRNAs in microtia and provides a basis for further investigation into their functional roles and underlying mechanisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Largo no Codificante / Microtia Congénita Límite: Humans Idioma: En Revista: Genet Test Mol Biomarkers Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Largo no Codificante / Microtia Congénita Límite: Humans Idioma: En Revista: Genet Test Mol Biomarkers Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos