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Influence of Different Photobiomodulation Parameters on Multi-Potent Adipose Tissue Mesenchymal Cells In Vitro.
Ferro, Ana Paula; de Jesus Guirro, Rinaldo Roberto; Ferraresi, Cleber; Celli, Jonathan; Orellana, Maristela Delgado; de Santis, Gil Cunha; Junior, Jayme Adriano Farina; de Oliveira Guirro, Elaine Caldeira.
Afiliación
  • Ferro AP; Department of Health Sciences, Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • de Jesus Guirro RR; Department of Health Sciences, Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • Ferraresi C; Department of Physical Therapy, Postgraduate Program in Physiotherapy, Federal University of São Carlos, São Paulo, Brazil.
  • Celli J; Department of Physics, University of Massachusetts Boston, Boston, Massachusetts, USA.
  • Orellana MD; Department of Cell Biology, Ribeirão Preto Blood Center Foundation, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • de Santis GC; Department of Cell Therapy, Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • Junior JAF; Department of Surgery, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • de Oliveira Guirro EC; Department of Health Sciences, Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Photobiomodul Photomed Laser Surg ; 42(3): 200-207, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38416634
ABSTRACT

Objective:

Investigating the effect of different parameters of photobiomodulation (PBM) with low-power laser on multi-potent mesenchymal stem cells (MSCs) derived from adipose tissue in terms of proliferation and cell death.

Methods:

MSCs were submitted to PBM applications with combinations of the following physical parameters control group (no intervention), wavelengths of 660 and 830 nm; energy of 0.5, 2, and 4 J; and power of 40 and 100 mW. MSC analysis was performed using MetaXpress® software at 24, 48, and 72 h.

Results:

Irradiation promoted a significant increase in cell proliferation (p < 0.05), with 830 nm laser, 100 mW, with energy of 0.5, 2, and 4 J in relation to the control group at all times. PBM with 660 nm, power of 40 mW, and energy of 0.5, 2, and 4 J produced greater cell death at 24 h compared with the control group. At the time of 72 h, there was no significant difference concerning cell death.

Conclusions:

According to the results found, we can conclude that both wavelengths were effective; however, the 830 nm laser was more effective in terms of cell proliferation compared with the 660 nm laser. The 660 nm wavelength showed a significant increase in cell death when compared with the 830 nm laser.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terapia por Luz de Baja Intensidad / Células Madre Mesenquimatosas Idioma: En Revista: Photobiomodul Photomed Laser Surg Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terapia por Luz de Baja Intensidad / Células Madre Mesenquimatosas Idioma: En Revista: Photobiomodul Photomed Laser Surg Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos