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Cryopreservation of sperm from the gudgeon, Microphysogobio rapidus (Cyprinidae): Effects of cryoprotectant, diluents, and dilution ratio.
Zidni, Irfan; Kim, Kang-Won; Jang, Hyun-Seok; Heo, Moon-Seong; Kim, Keun-Sik; Yoon, Ju-Duk; Lim, Han-Kyu.
Afiliación
  • Zidni I; Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Mokpo National University, Republic of Korea; Department of Fisheries, The Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency, 45363, Indonesia.
  • Kim KW; Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Mokpo National University, Republic of Korea.
  • Jang HS; Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Mokpo National University, Republic of Korea.
  • Heo MS; National Institute of Ecology, Gyeongsangbuk-do, 36531, Republic of Korea.
  • Kim KS; National Institute of Ecology, Gyeongsangbuk-do, 36531, Republic of Korea.
  • Yoon JD; National Institute of Ecology, Gyeongsangbuk-do, 36531, Republic of Korea.
  • Lim HK; Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Mokpo National University, Republic of Korea; Department of Marine and Fisheries Resources, Mokpo National University, Republic of Korea. Electronic address: limhk@mokpo.ac.kr.
Cryobiology ; 115: 104869, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38412911
ABSTRACT
We investigated methods for cryopreserving sperm from the endangered gudgeon, Microphysogobio rapidus, by examining the effects of cryoprotective agent (CPA) concentration, diluent, and dilution ratio on post-thaw sperm quality. The quality of frozen sperm was evaluated in terms of motility and kinematic parameters, viability, DNA damage, and fertilization rate. We evaluated methanol, glycerol, dimethyl sulfoxide (DMSO), and ethylene glycol as CPAs. Sperm motility, velocity, and viability were significantly higher when methanol was used as the CPA (p < 0.05). The diluents tested were Ringer's solution, Kurokura's Extender, Common Carp Sperm Extender (CCSE), and buffered sperm motility-inhibiting saline solution (BSMIS); post-thaw motility was highest when Ringer's solution was used as the diluent. Next, various quantities of methanol were combined with Ringer's solution to identify the optimal dose of methanol. The dilution ratios tested ranged from 11 to 17. Cryopreserved sperm was thawed at 20 °C for 15 s. The use of 10% methanol with Ringer's solution at a dilution ratio of 15 resulted in the highest post-thaw sperm motility, viability, and velocity including VAP, VCL, and VSL. Post-thaw sperm showed significantly greater DNA damage than the control (fresh sperm) (p < 0.05). The fertilization rate was highest with fresh sperm (p < 0.05), followed by sperm frozen with 10% methanol + Ringer's solution. We recommend that the best way to preserve sperm in the studied species is to use a combination of Ringer's solution and 10% methanol at a 15 dilution ratio. Our findings will facilitate the artificial fertilization of M. rapidus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Motilidad Espermática / Espermatozoides / Cyprinidae / Criopreservación / Dimetilsulfóxido / Crioprotectores / Metanol Límite: Animals Idioma: En Revista: Cryobiology Año: 2024 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Motilidad Espermática / Espermatozoides / Cyprinidae / Criopreservación / Dimetilsulfóxido / Crioprotectores / Metanol Límite: Animals Idioma: En Revista: Cryobiology Año: 2024 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Países Bajos