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In vitro regeneration of triploid from mature endosperm culture of Passiflora edulis "Mantianxing".
Tang, Junrong; Luo, Xi; Zhu, Yajing; Cai, Nianhui; Chen, Lin; Chen, Shi; Xu, Yulan.
Afiliación
  • Tang J; Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, China.
  • Luo X; College of Forestry, Southwest Forestry University, Kunming, China.
  • Zhu Y; Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, China.
  • Cai N; College of Forestry, Southwest Forestry University, Kunming, China.
  • Chen L; Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, China.
  • Chen S; College of Forestry, Southwest Forestry University, Kunming, China.
  • Xu Y; Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, China.
Biosci Biotechnol Biochem ; 88(4): 412-419, 2024 Mar 22.
Article en En | MEDLINE | ID: mdl-38412471
ABSTRACT
The regeneration of shoots from endosperm tissue is a highly effective method to obtain triploid plants. In this study, we elucidated the establishment of an in vitro regeneration system from endosperm culture for the production of Passiflora edulis "Mantianxing." The highest callus induction rate (83.33%) was obtained on the media supplemented with 1.0 mg/L TDZ. Meanwhile, the MS medium containing 1.0 mg/L 6-BA and 0.4 mg/L IBA gave the optimum 75% shoot bud induction. Chromosome analysis revealed that the chromosomal count of P. edulis "Mantianxing" regenerated from endosperm tissues was 27 (2n = 3x = 27), which indicated that shoots regenerated from endosperm tissues were triploids. Triploid P. edulis had more drought resistance than diploid plants. Our study provided a method for breeding of passion fruit by means of a stable and reproducible regeneration system from endosperm culture, leading to the generation of triploid plants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Passiflora / Triploidía Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Passiflora / Triploidía Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido