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Novel formulation of parthenolide-loaded liposome coated with chitosan and evaluation of its potential anticancer effects in vitro.
Ensaf, Parisa Karimian; Goodarzi, Mohammad Taghi; Tabrizi, Masoud Homayouni; Neamati, Ali; Hosseinyzadeh, Samira Sadat.
Afiliación
  • Ensaf PK; Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
  • Goodarzi MT; Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran. mtgoodarzi@yahoo.com.
  • Tabrizi MH; Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
  • Neamati A; Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
  • Hosseinyzadeh SS; Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
Mol Biol Rep ; 51(1): 369, 2024 Feb 27.
Article en En | MEDLINE | ID: mdl-38411765
ABSTRACT

BACKGROUND:

In this study the formulation of parthenolide (PN), an anticancer agent extracted from a natural product, into a liposome (PN-liposome), was examined. The surface of the PN-liposome was modified using chitosan (PN-chitosome). By using real-time quantitative PCR and flow cytometry, we examined the release of PN-chitosomes, cytotoxicity, and ability to induce apoptosis in vitro. METHODS AND

RESULTS:

According to the present study, PN-chitosomes had a size of 251 nm which is acceptable for efficient enhanced permeation and retention (EPR) performance. PN-chitosomes were confirmed to be spherical in shape and size through FESEM analysis. In terms of encapsulation efficiency, 94.5% was achieved. PN-chitosome possessed a zeta potential of 34.72 mV, which was suitable for its stability. According to the FTIR spectra of PN and PN-chitosome, PN was chemically stable due to the intermolecular interaction between the liposome and the drug. After 48 h, only 10% of the PN was released from the PN-chitosome in PBS (pH 7.4), and less than 20% was released after 144 h.

CONCLUSION:

In a dose-dependent manner, PN-chitosome exhibited anticancer properties that were more cytotoxic against cancer cells than normal cells. Moreover, the formulation activated both the apoptosis pathway and cytotoxic genes in real-time qPCR experiments. According to the cytotoxicity and activating apoptosis of the prepared modified particle, PN-chitosome may be helpful in the treatment of cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Quitosano Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Quitosano Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos