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Aerobic Exercise Attenuates Pressure Overload-Induced Myocardial Remodeling and Myocardial Inflammation via Upregulating miR-574-3p in Mice.
Chen, Qiao-Ying; Jiang, Yi-Na; Guan, Xuan; Ren, Fang-Fang; Wu, Shu-Jie; Chu, Mao-Ping; Wu, Lian-Pin; Lai, Teng-Fang; Li, Lei.
Afiliación
  • Chen QY; Department of Cardiology, Key Laboratory of Panvascular Diseases of Wenzhou, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Jiang YN; Key Laboratory of Structural Malformations in Children of Zhejiang Province, Wenzhou, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Guan X; Department of Cardiology, Key Laboratory of Panvascular Diseases of Wenzhou, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Ren FF; Key Laboratory of Structural Malformations in Children of Zhejiang Province, Wenzhou, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Wu SJ; Department of Cardiology, Key Laboratory of Panvascular Diseases of Wenzhou, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Chu MP; Key Laboratory of Structural Malformations in Children of Zhejiang Province, Wenzhou, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Wu LP; Department of Cardiology, Key Laboratory of Panvascular Diseases of Wenzhou, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Lai TF; Key Laboratory of Structural Malformations in Children of Zhejiang Province, Wenzhou, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
  • Li L; Department of Cardiology, Key Laboratory of Panvascular Diseases of Wenzhou, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, China (Q.-Y.C., Y.-N.J., X.G., F.-F.R., S.-J.W., M.-P.C., L.-P.W., L.L.).
Circ Heart Fail ; 17(3): e010569, 2024 03.
Article en En | MEDLINE | ID: mdl-38410978
ABSTRACT

BACKGROUND:

Exercise training can promote cardiac rehabilitation, thereby reducing cardiovascular disease mortality and hospitalization rates. MicroRNAs (miRs) are closely related to heart disease, among which miR-574-3p plays an important role in myocardial remodeling, but its role in exercise-mediated cardioprotection is still unclear.

METHODS:

A mouse myocardial hypertrophy model was established by transverse aortic coarctation, and a 4-week swimming exercise training was performed 1 week after the operation. After swimming training, echocardiography was used to evaluate cardiac function in mice, and histopathologic staining was used to detect cardiac hypertrophy, myocardial fibrosis, and cardiac inflammation. Quantitative real-time polymerase chain reaction was used to detect the expression levels of miR-574-3p and cardiac hypertrophy markers. Western blotting detected the IL-6 (interleukin-6)/JAK/STAT inflammatory signaling pathway.

RESULTS:

Echocardiography and histochemical staining found that aerobic exercise significantly improved pressure overload-induced myocardial hypertrophy (n=6), myocardial interstitial fibrosis (n=6), and cardiac inflammation (n=6). Quantitative real-time polymerase chain reaction detection showed that aerobic exercise upregulated the expression level of miR-574-3p (n=6). After specific knockdown of miR-574-3p in mouse hearts with adeno-associated virus 9 using cardiac troponin T promoter, we found that the protective effect of exercise training on the heart was significantly reversed. Echocardiography and histopathologic staining showed that inhibiting the expression of miR-574-3p could partially block the effects of aerobic exercise on cardiac function (n=6), cardiomyocyte cross-sectional area (n=6), and myocardial fibrosis (n=6). Western blotting and immunohistochemical staining showed that the inhibitory effects of aerobic exercise on the IL-6/JAK/STAT pathway and cardiac inflammation were partially abolished after miR-574-3p knockdown. Furthermore, we also found that miR-574-3p exerts cardioprotective effects in cardiomyocytes by targeting IL-6 (n=3).

CONCLUSIONS:

Aerobic exercise protects cardiac hypertrophy and inflammation induced by pressure overload by upregulating miR-574-3p and inhibiting the IL-6/JAK/STAT pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Insuficiencia Cardíaca / Miocarditis Límite: Animals Idioma: En Revista: Circ Heart Fail Asunto de la revista: ANGIOLOGIA / CARDIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Insuficiencia Cardíaca / Miocarditis Límite: Animals Idioma: En Revista: Circ Heart Fail Asunto de la revista: ANGIOLOGIA / CARDIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos