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Single-cell RNA sequencing reveals roles of unique retinal microglia types in early diabetic retinopathy.
Wang, Yan; Yang, Xiongyi; Zhang, Yuxi; Hong, Libing; Xie, Zhuohang; Jiang, Wenmin; Chen, Lin; Xiong, Ke; Yang, Siyu; Lin, Meiping; Guo, Xi; Li, Qiumo; Deng, Xiaoqing; Lin, Yanhui; Cao, Mingzhe; Yi, Guoguo; Fu, Min.
Afiliación
  • Wang Y; Department of Ophthalmology, South China Hospital, Medical School, Shenzhen University, Shenzhen, 518116, People's Republic of China.
  • Yang X; The Second Clinical School, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
  • Zhang Y; State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Guangdong Provincial Institute of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
  • Hong L; The Second Clinical School, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
  • Xie Z; The Second Clinical School, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
  • Jiang W; Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, People's Republic of China.
  • Chen L; Hunan Clinical Research Center of Ophthalmic Disease, Changsha, 410011, Hunan, People's Republic of China.
  • Xiong K; Department of Anesthesiology, Shenzhen Hospital, Southern Medical University, 1333 Xinhu Road, Shenzhen, 518100, Guangdong, People's Republic of China.
  • Yang S; Department of Ophthalmology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
  • Lin M; Department of Ophthalmology, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, People's Republic of China.
  • Guo X; The Second Clinical School, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
  • Li Q; School of Rehabilitation Medicine, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
  • Deng X; The Second Clinical School, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
  • Lin Y; The Second Clinical School, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
  • Cao M; Health Management Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, People's Republic of China.
  • Yi G; Department of Ophthalmology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China. caomzh7@mail.sysu.edu.cn.
  • Fu M; Department of Ophthalmology, The Sixth Affiliated Hospital, Sun Yat-Sen University, No. 26, Erheng Road, Yuancun, Tianhe, Guangzhou, Guangdong, People's Republic of China. yigg@mail.sysu.edu.cn.
Diabetol Metab Syndr ; 16(1): 49, 2024 Feb 26.
Article en En | MEDLINE | ID: mdl-38409074
ABSTRACT

BACKGROUND:

The pathophysiological mechanisms of diabetic retinopathy (DR), a blinding disease, are intricate. DR was thought to be a microvascular disease previously. However, growing studies have indicated that the retinal microglia-induced inflammation precedes microangiopathy. The binary concept of microglial M1/M2 polarization paradigms during inflammatory activation has been debated. In this study, we confirmed microglia had the most significant changes in early DR using single-cell RNA sequencing.

METHODS:

A total of five retinal specimens were collected from donor SD rats. Changes in various cells of the retina at the early stage of DR were analyzed using single-cell sequencing technology.

RESULTS:

We defined three new microglial subtypes at cellular level, including two M1 types (Egr2+ M1 and Egr2- M1) and one M2 type. We also revealed the anatomical location between these subtypes, the dynamic changes of polarization phenotypes, and the possible activation sequence and mutual activation regulatory mechanism of different cells. Furthermore, we constructed an inflammatory network involving microglia, blood-derived macrophages and other retinal nonneuronal cells. The targeted study of new disease-specific microglial subtypes can shorten the time for drug screening and clinical application, which provided insight for the early control and reversal of DR.

CONCLUSIONS:

We found that microglia show the most obvious differential expression changes in early DR and reveal the changes in microglia in a high-glucose microenvironment at the single-cell level. Our comprehensive analysis will help achieve early reversal and control the occurrence and progression of DR.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Diabetol Metab Syndr Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Diabetol Metab Syndr Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido