Your browser doesn't support javascript.
loading
A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions.
Farrell, Ryan J; Bredvik, Kirsten G; Hoppa, Michael B; Hennigan, S Thomas; Brown, Timothy A; Ryan, Timothy A.
Afiliación
  • Farrell RJ; Department of Biochemistry, Weill Cornell Medicine, New York, NY, USA, 10065.
  • Bredvik KG; David Rockefeller Graduate Program, The Rockefeller University, New York, NY, USA, 10065.
  • Hoppa MB; Present Address: Neuroscience Institute, NYU Medical Center, New York, NY, USA, 10016.
  • Hennigan ST; Department of Biochemistry, Weill Cornell Medicine, New York, NY, USA, 10065.
  • Brown TA; Tri-Institutional M.D./Ph.D. Program, Weill Cornell Medicine, New York, NY, USA, 10065.
  • Ryan TA; Department of Biochemistry, Weill Cornell Medicine, New York, NY, USA, 10065.
bioRxiv ; 2024 Feb 16.
Article en En | MEDLINE | ID: mdl-38405980
ABSTRACT
The endoplasmic reticulum (ER) is an important regulator of Ca2+ in cells and dysregulation of ER calcium homeostasis can lead to numerous pathologies. Understanding how various pharmacological and genetic perturbations of ER Ca2+ homeostasis impacts cellular physiology would likely be facilitated by more quantitative measurements of ER Ca2+ levels that allow easier comparisons across conditions. Here, we developed a ratiometric version of our original ER-GCaMP probe that allows for more quantitative comparisons of the concentration of Ca2+ in the ER across cell types and sub-cellular compartments. Using this approach we show that the resting concentration of ER Ca2+ in primary dissociated neurons is substantially lower than that in measured in embryonic fibroblasts.
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos