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Fusing Peptide Epitopes for Advanced Multiplex Serological Testing for SARS-CoV-2 Antibody Detection.
Aldoukhi, Ali H; Bilalis, Panayiotis; Alhattab, Dana M; Valle-Pérez, Alexander U; Susapto, Hepi H; Pérez-Pedroza, Rosario; Backhoff-García, Emiliano; Alsawaf, Sarah M; Alshehri, Salwa; Boshah, Hattan; Alrashoudi, Abdulelah A; Aljabr, Waleed A; Alaamery, Manal; Alrashed, May; Hasanato, Rana M; Farzan, Raed A; Alsubki, Roua A; Moretti, Manola; Abedalthagafi, Malak S; Hauser, Charlotte A E.
Afiliación
  • Aldoukhi AH; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Bilalis P; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Alhattab DM; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Valle-Pérez AU; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Susapto HH; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Pérez-Pedroza R; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Backhoff-García E; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Alsawaf SM; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Alshehri S; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Boshah H; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Alrashoudi AA; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Aljabr WA; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Alaamery M; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Alrashed M; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Hasanato RM; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Farzan RA; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Alsubki RA; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Moretti M; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Abedalthagafi MS; Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal 23955-69900, Saudi Arabia.
  • Hauser CAE; Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
ACS Bio Med Chem Au ; 4(1): 37-52, 2024 Feb 21.
Article en En | MEDLINE | ID: mdl-38404747
ABSTRACT
The tragic COVID-19 pandemic, which has seen a total of 655 million cases worldwide and a death toll of over 6.6 million seems finally tailing off. Even so, new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue to arise, the severity of which cannot be predicted in advance. This is concerning for the maintenance and stability of public health, since immune evasion and increased transmissibility may arise. Therefore, it is crucial to continue monitoring antibody responses to SARS-CoV-2 in the general population. As a complement to polymerase chain reaction tests, multiplex immunoassays are elegant tools that use individual protein or peptide antigens simultaneously to provide a high level of sensitivity and specificity. To further improve these aspects of SARS-CoV-2 antibody detection, as well as accuracy, we have developed an advanced serological peptide-based multiplex assay using antigen-fused peptide epitopes derived from both the spike and the nucleocapsid proteins. The significance of the epitopes selected for antibody detection has been verified by in silico molecular docking simulations between the peptide epitopes and reported SARS-CoV-2 antibodies. Peptides can be more easily and quickly modified and synthesized than full length proteins and can, therefore, be used in a more cost-effective manner. Three different fusion-epitope peptides (FEPs) were synthesized and tested by enzyme-linked immunosorbent assay (ELISA). A total of 145 blood serum samples were used, compromising 110 COVID-19 serum samples from COVID-19 patients and 35 negative control serum samples taken from COVID-19-free individuals before the outbreak. Interestingly, our data demonstrate that the sensitivity, specificity, and accuracy of the results for the FEP antigens are higher than for single peptide epitopes or mixtures of single peptide epitopes. Our FEP concept can be applied to different multiplex immunoassays testing not only for SARS-CoV-2 but also for various other pathogens. A significantly improved peptide-based serological assay may support the development of commercial point-of-care tests, such as lateral-flow-assays.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Bio Med Chem Au Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Bio Med Chem Au Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos