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Identification of lipid synthesis genes in Schizochytrium sp. and their application in improving eicosapentaenoic acid synthesis in Yarrowia lipolytica.
Jia, Yu-Lei; Zhang, Qing-Ming; Du, Fei; Yang, Wen-Qian; Zhang, Zi-Xu; Xu, Ying-Shuang; Ma, Wang; Sun, Xiao-Man; Huang, He.
Afiliación
  • Jia YL; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
  • Zhang QM; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
  • Du F; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
  • Yang WQ; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
  • Zhang ZX; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
  • Xu YS; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
  • Ma W; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
  • Sun XM; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China. xiaomansun@njnu.edu.cn.
  • Huang H; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210000, China.
Biotechnol Biofuels Bioprod ; 17(1): 32, 2024 Feb 24.
Article en En | MEDLINE | ID: mdl-38402213
ABSTRACT

BACKGROUND:

Eicosapentaenoic acid (EPA) is widely used in the functional food and nutraceutical industries due to its important benefits to human health. Oleaginous microorganisms are considered a promising alternative resource for the production of EPA lipids. However, the storage of EPA in triglyceride (TG) becomes a key factor limiting its level.

RESULTS:

This study aimed to incorporate more EPA into TG storage through metabolic engineering. Firstly, key enzymes for TG synthesis, the diacylglycerol acyltransferase (DGAT) and glycerol-3-phosphate acyltransferase (GPAT) genes from Schizochytrium sp. HX-308 were expressed in Yarrowia lipolytica to enhance lipid and EPA accumulation. In addition, engineering the enzyme activity of DGATs through protein engineering was found to be effective in enhancing lipid synthesis by replacing the conserved motifs "HFS" in ScDGAT2A and "FFG" in ScDGAT2B with the motif "YFP". Notably, combined with lipidomic analysis, the expression of ScDGAT2C and GPAT2 enhanced the storage of EPA in TG. Finally, the accumulation of lipid and EPA was further promoted by identifying and continuing to introduce the ScACC, ScACS, ScPDC, and ScG6PD genes from Schizochytrium sp., and the lipid and EPA titer of the final engineered strain reached 2.25 ± 0.03 g/L and 266.44 ± 5.74 mg/L, respectively, which increased by 174.39% (0.82 ± 0.02 g/L) and 282.27% (69.70 ± 0.80 mg/L) compared to the initial strain, respectively.

CONCLUSION:

This study shows that the expression of lipid synthesis genes from Schizochytrium sp. in Y. lipolytica effectively improves the synthesis of lipids and EPA, which provided a promising target for EPA-enriched microbial oil production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biotechnol Biofuels Bioprod Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biotechnol Biofuels Bioprod Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido