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Evaluation of the Antibacterial Activity of New Dermaseptin Derivatives against Acinetobacter baumannii.
Haddad, Houda; Mejri, Radhia; de Araujo, Alyne Rodrigues; Zaïri, Amira.
Afiliación
  • Haddad H; BIOLIVAL Laboratory, LR14ES06, The Higher Institute of Biotechnology of Monastir ISBM, University of Monastir, Monastir 5000, Tunisia.
  • Mejri R; Biochemistry Department, LR18ES47, Faculty of Medicine, University of Sousse, Sousse 4002, Tunisia.
  • de Araujo AR; Biochemistry Department, LR18ES47, Faculty of Medicine, University of Sousse, Sousse 4002, Tunisia.
  • Zaïri A; Biodiversity and Biotechnology Research Center, BIOTEC, Federal University of Piauí, Parnaíba 64202-020, PI, Brazil.
Pharmaceuticals (Basel) ; 17(2)2024 Jan 29.
Article en En | MEDLINE | ID: mdl-38399385
ABSTRACT
Nosocomial infections represent one of the biggest health problems nowadays. Acinetobacter baumannii is known as an opportunistic pathogen in humans, affecting people with compromised immune systems, and is becoming increasingly important as a hospital-derived infection. It is known that in recent years, more and more bacteria have become multidrug-resistant (MDR) and, for this reason, the development of new drugs is a priority. However, these products must not affect the human body, and therefore, cytotoxicity studies are mandatory. In this context, antimicrobial peptides with potential antibacterial proprieties could be an alternative. In this research, we describe the synthesis and the bioactivity of dermaseptins and their derivatives against Acinetobacter baumannii. The cytotoxicity of these compounds was investigated on the HEp-2 cell line by MTT cell viability assay. Thereafter, we studied the morphological alterations caused by the action of one of the active peptides on the bacterial membrane using atomic force microscopy (AFM). The cytotoxicity of dermaseptins was concentration-dependent at microgram concentrations. It was observed that all tested analogs exhibited antibacterial activity with Minimum Inhibitory Concentrations (MICs) ranging from 3.125 to 12.5 µg/mL and Minimum Bactericidal Concentrations (MBCs) ranging from 6.25 to 25 µg/mL. Microscopic images obtained by AFM revealed morphological changes on the surface of the treated bacteria caused by K4S4(1-16), as well as significant surface alterations. Overall, these findings demonstrate that dermaseptins might constitute new lead structures for the development of potent antibacterial agents against Acinetobacter baumannii infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Suiza