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An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells.
Jahun, Aminu S; Sorgeloos, Frederic; Goodfellow, Ian G.
Afiliación
  • Jahun AS; Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital Level 5, Hills Road, CB2 0QQ Cambridge, UK. Electronic address: asj40@cam.ac.uk.
  • Sorgeloos F; Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital Level 5, Hills Road, CB2 0QQ Cambridge, UK; Université catholique de Louvain, de Duve Institute, MIPA-VIRO 74-49, 74 Avenue Hippocrate, 1200 Brussels, Belgium.
  • Goodfellow IG; Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital Level 5, Hills Road, CB2 0QQ Cambridge, UK. Electronic address: ig299@cam.ac.uk.
STAR Protoc ; 5(1): 102913, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38393950
ABSTRACT
Leakage of mitochondrial or nuclear DNA into the cytosol can occur following viral infections, radiation damage, and some cancers. Here, we present an optimized protocol for isolating and quantifying cytosolic DNA from mammalian cells. We describe steps for collecting cytosolic fractions from cells, extracting DNA using columns, and quantifying extracted DNA using qPCR. This straightforward protocol can be completed in as little as 5 hours, and allows for the identification of the source of DNA. For complete details on the use and execution of this protocol, please refer to Jahun et al.1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Mitocondrias Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Mitocondrias Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos