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Studying Translesion DNA Synthesis Using Xenopus In Vitro Systems.
Aze, Antoine; Hutchins, James R A; Maiorano, Domenico.
Afiliación
  • Aze A; Genome Surveillance and Stability Laboratory, Institute of Human Genetics, UMR9002, CNRS-University of Montpellier, Montpellier, France.
  • Hutchins JRA; Genome Surveillance and Stability Laboratory, Institute of Human Genetics, UMR9002, CNRS-University of Montpellier, Montpellier, France.
  • Maiorano D; Genome Surveillance and Stability Laboratory, Institute of Human Genetics, UMR9002, CNRS-University of Montpellier, Montpellier, France. domenico.maiorano@igh.cnrs.fr.
Methods Mol Biol ; 2740: 21-36, 2024.
Article en En | MEDLINE | ID: mdl-38393467
ABSTRACT
Cell-free extracts derived from Xenopus eggs have been widely used to decipher molecular pathways involved in several cellular processes including DNA synthesis, the DNA damage response, and genome integrity maintenance. We set out assays using Xenopus cell-free extracts to study translesion DNA synthesis (TLS), a branch of the DNA damage tolerance pathway that allows replication of damaged DNA. Using this system, we were able to recapitulate TLS activities that occur naturally in vivo during early embryogenesis. This chapter describes protocols to detect chromatin-bound TLS factors by western blotting and immunofluorescence microscopy upon induction of DNA damage by UV irradiation, monitor TLS-dependent mutagenesis, and perform proteomic screening.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteómica / Síntesis Translesional de ADN Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteómica / Síntesis Translesional de ADN Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos