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CircRNA-5335 Regulates the Differentiation and Proliferation of Sheep Preadipocyte via the miR-125a-3p/STAT3 Pathway.
Guo, Wei; Ciwang, Renzeng; Wang, Lei; Zhang, Shuer; Liu, Nan; Zhao, Jinshan; Zhou, Lisheng; Li, Hegang; Gao, Xiaoxiao; He, Jianning.
Afiliación
  • Guo W; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
  • Ciwang R; Institute of Animal Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850009, China.
  • Wang L; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
  • Zhang S; Shandong Animal Husbandry Chief Station, Jinan 250100, China.
  • Liu N; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
  • Zhao J; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
  • Zhou L; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
  • Li H; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
  • Gao X; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
  • He J; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
Vet Sci ; 11(2)2024 Feb 04.
Article en En | MEDLINE | ID: mdl-38393088
ABSTRACT
The content of intramuscular fat (IMF) from preadipocytes is proportional to meat quality in livestock. However, the roles of circRNAs in IMF deposition in sheep are not well known. In this study, we show that circRNA-5335/miR-125a-3p/STAT3 play a crucial adjective role in the proliferation and differentiation of sheep preadipocytes. In this study, we characterized the roles of differentially expressed circRNA-5335/miR-125a-3p/STAT3, which were screened from sheep of different months of age and based on sequencing data. Firstly, the expression profiles of circRNA-5335/miR-125a-3p/STAT3 were identified during the differentiation of preadipocytes in vitro by RT-qPCR and WB. Then, the targeting relationship of the circRNA-5335/miR-125a-3p/STAT3 was verified by dual-luciferase reporter assays. The results of RT-qPCR, CCK8, EdU and Oil Red O staining assay showed that miR-125a-3p suppressed the differentiation and raised the proliferation of preadipocytes by targeting STAT3. As a competing endogenous RNA, the downregulation of circRNA-5335 decreased the expression of STAT3 by increasing miR-125a-3p, which inhibited the differentiation of preadipocytes and promoted proliferation. Our present study demonstrates the functional significance of circRNA-5335/miR-125a-3p/STAT3 in the differentiation of sheep preadipocytes, and provides novel insights into exploring the mechanism of IMF.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza