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Characterization of Three Types of Elongases from Different Fungi and Site-Directed Mutagenesis.
Wang, Yuxin; Chang, Lulu; Zhang, Hao; Chen, Yong Q; Chen, Wei; Chen, Haiqin.
Afiliación
  • Wang Y; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
  • Chang L; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
  • Zhang H; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
  • Chen YQ; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
  • Chen W; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
  • Chen H; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
J Fungi (Basel) ; 10(2)2024 Feb 03.
Article en En | MEDLINE | ID: mdl-38392800
ABSTRACT
Fatty acid elongases play crucial roles in synthesizing long-chain polyunsaturated fatty acids. Identifying more efficient elongases is essential for enhancing oleaginous microorganisms to produce high yields of target products. We characterized three elongases that were identified with distinct specificities McELO from Mucor circinelloides, PrELO from Phytophthora ramorum, and PsELO from Phytophthora sojae. Heterologous expression in Saccharomyces cerevisiae showed that McELO preferentially elongates C16 to C18 fatty acids, PrELO targets Δ6 polyunsaturated fatty acids, and PsELO uses long chain saturated fatty acids as substrates. McELO and PrELO exhibited more homology, potentially enabling fatty acid composition remodeling and enhanced LC-PUFAs production in oleaginous microorganisms. Site-directed mutagenesis of conserved amino acids across elongase types identified residues essential for activity, supported by molecular docking. Alanine substitution of conserved polar residues led to enzyme inactivation, underscoring their importance in the condensation reaction. Our findings offer promising elongase candidates for polyunsaturated fatty acid production, contributing to the bioindustry's sustainable development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza