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Enniatin B1 induces damage to Leydig cells via inhibition of the Nrf2/HO-1 and JAK/STAT3 signaling pathways.
Shen, Hongping; Cai, Yili; Zhu, Keqi; Wang, Dong; Yu, Rui; Chen, Xueqin.
Afiliación
  • Shen H; Department of Traditional Chinese Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang Province, China.
  • Cai Y; Department of Acupuncture, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang Province, China.
  • Zhu K; Department of Traditional Chinese Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang Province, China.
  • Wang D; Shanghai Houyu Medical Equiment Co., Ltd, China.
  • Yu R; Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningbo University, China. Electronic address: yurui@nbu.edu.cn.
  • Chen X; Department of Traditional Chinese Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang Province, China. Electronic address: cxq2316@163.com.
Ecotoxicol Environ Saf ; 273: 116116, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38387140
ABSTRACT
Enniatin B1 (ENN B1) is a mycotoxin that can be found in various foods. However, whether ENN B1 is hazardous to the reproductive system is still elusive. Leydig cells are testosterone-generating cells that reside in the interstitial compartment between seminiferous tubules. Dysfunction of Leydig cells could result in male infertility. This study aimed to examine the toxicological effects of ENN B1 against TM3 Leydig cells. ENN B1 significantly inhibited cell viability in a dose-dependent manner. ENN B1 treatment also decreased the expression of functional genes in Leydig cells. Moreover, ENN B1 induced Leydig cells apoptosis and oxidative stress. Mechanistically, ENN B1 leads to the upregulation of Bax and downregulation of Bcl-2 in Leydig cells. In addition, ENN B1 inhibited the Nrf2/HO-1 pathway, which is critical for the induction of oxidative stress. Additionally, ENN B1 treatment repressed the JAK/STAT3 signaling pathway in Leydig cells. Rescue experiments showed that activation of STAT3 resulted in alleviation of ENN B1-induced damage in Leydig cells. Collectively, our study demonstrated that ENN B1 induced Leydig cell dysfunction via multiple mechanisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Depsipéptidos / Células Intersticiales del Testículo / Micotoxinas Límite: Humans / Male Idioma: En Revista: Ecotoxicol Environ Saf Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Depsipéptidos / Células Intersticiales del Testículo / Micotoxinas Límite: Humans / Male Idioma: En Revista: Ecotoxicol Environ Saf Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos