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Elevated KDM4D Expression in Pterygium: Impact and Potential Inhibition by Lycium Barbarum Polysaccharide.
Gao, Juan; Wang, Yuchuan; Han, Ruifang; Li, Jun; Hao, Peng; Li, Jing; Chen, Xi; Jiang, Li; Wang, Liming; Ma, Yunqing; Chen, Luxia; Li, Xuan.
Afiliación
  • Gao J; Tianjin Eye Hospital, Tianjin, China.
  • Wang Y; Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin, China.
  • Han R; Tianjin Eye Institute, Tianjin, China.
  • Li J; Tianjin Eye Hospital, Tianjin, China.
  • Hao P; Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin, China.
  • Li J; Tianjin Eye Institute, Tianjin, China.
  • Chen X; Tianjin Eye Hospital, Tianjin, China.
  • Jiang L; Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin, China.
  • Wang L; Tianjin Eye Institute, Tianjin, China.
  • Ma Y; Tianjin Eye Hospital, Tianjin, China.
  • Chen L; Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin, China.
  • Li X; Tianjin Eye Institute, Tianjin, China.
J Ocul Pharmacol Ther ; 40(3): 181-188, 2024 04.
Article en En | MEDLINE | ID: mdl-38386983
ABSTRACT

Purpose:

This study aimed to explore the effects of elevated KDM4D expression and potential therapeutic effects of Lycium barbarum polysaccharide (LBP) on pterygium.

Methods:

The expression levels of KDM4D in the primary pterygium (n = 29) and normal conjunctiva (n = 14) were detected by immunohistochemistry. The effects of KDM4D on pterygium fibroblasts were detected by the CCK-8 assay, liquid chromatography-mass spectrometry assay, flow cytometry, and scratch wound healing assay. The relative expression of KDM4D in pterygium fibroblasts stimulated by interleukin (IL)-1ß, IL-6, IL-8, and LBP was detected by quantitative real-time PCR and Western blot. The effects of LBP on pterygium fibroblasts were detected using flow cytometry and scratch wound healing assays.

Results:

The expression level of KDM4D in pterygium was higher than that in normal conjunctiva. KDM4D increased the cell viability of pterygium fibroblasts. The differentially expressed genes identified in the LM-MS assay enriched in "actin filament organization" and "apoptosis." KDM4D promoted migration and inhibited apoptosis of pterygium fibroblasts in vitro. Inflammatory cytokines, including IL-1ß, IL-6, and IL-8, enhanced the expression of KDM4D in pterygium fibroblasts. LBP inhibited the expression of KDM4D in pterygium fibroblasts and decreased their cell viability. Moreover, LBP attenuated the KDM4D effects on migration and apoptosis of pterygium fibroblasts.

Conclusions:

Elevated KDM4D expression is a risk factor for pterygium formation. LBP inhibits the expression of KDM4D in pterygium fibroblasts and may be a potential drug for delaying pterygium development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pterigion / Medicamentos Herbarios Chinos / Conjuntiva Límite: Humans Idioma: En Revista: J Ocul Pharmacol Ther Asunto de la revista: FARMACOLOGIA / OFTALMOLOGIA / TERAPEUTICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pterigion / Medicamentos Herbarios Chinos / Conjuntiva Límite: Humans Idioma: En Revista: J Ocul Pharmacol Ther Asunto de la revista: FARMACOLOGIA / OFTALMOLOGIA / TERAPEUTICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos