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Impact of prolonged storage time on homograft ultrastructures: an attempt to find optimal guidelines for homograft processing.
von Konow, Ida; Eliasson, Angeline; Nilsson, Johan; Malm, Torsten.
Afiliación
  • von Konow I; Department of Cardiothoracic Surgery, Skane University Hospital, Lund, Sweden. Ida.von_konow@med.lu.se.
  • Eliasson A; Department of Clinical Sciences, Thoracic Surgery, Lund University, Lund, Sweden. Ida.von_konow@med.lu.se.
  • Nilsson J; Tissue Bank Lund, Skane University Hospital, Lund, Sweden. Ida.von_konow@med.lu.se.
  • Malm T; National Board of Forensic Medicine, Lund, Sweden.
Cell Tissue Bank ; 25(2): 649-662, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38386211
ABSTRACT
According to guidelines, total ischemic time for homografts at processing must be kept short to avoid degeneration. Many homografts are discarded due to practical inability to finish all steps from procurement to cryopreservation within the time limit. Although, several studies have shown that homografts with prolonged ischemic time show adequate quality and performance. Twenty aortic and 12 pulmonary homografts were collected and biopsies were retrieved at preparation (day 0) and after 1, 2, 3, 4, 7, 14, 21, 28, and 60 days in antibiotic decontamination at 4 °C. Biopsies were prepared for light microscopy (LM) and transmission electron microscopy (TEM). Assessment generated scores for cells, elastin, and collagen. Relative differences between times were compared with Wilcoxon signed rank test. Bonferroni corrected p value of 0.0056 was considered significant. LM could only reveal decrease in cell count at 60 days in aortic homografts, no other differences was detected. TEM showed affected cell appearance in day 3 and day 4 and beyond for aortic and pulmonary homografts respectively. Elastin appearance was affected at day 60 for aortic and day 21 for pulmonary homografts. Collagen appearance was affected at day 28 for aortic homografts, with no significant differences in pulmonary homografts. Cell degeneration starts early after homograft procurement, but elastic and collagen fibers are more resistant to degeneration. Overall structure integrity as seen in LM was not affected at all, while TEM could reveal small degeneration signs in individual elastic fibers and collagen bundles at 21 and 28 days respectively.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aorta / Aloinjertos Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aorta / Aloinjertos Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Países Bajos