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Oral Lactobacillus zeae exacerbates the pathological manifestation of periodontitis in a mouse model.
Chen, Yi-Wen; Hou, Yu-Wen; Wang, Chuang-Wei; Cheng, Shih-Jung; Kuo, Wei-Ting; Lin, Chun-Pin; Hou, Hsin-Han.
Afiliación
  • Chen YW; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
  • Hou YW; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
  • Wang CW; Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
  • Cheng SJ; Department of Dermatology, Drug Hypersensitivity Clinical and Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
  • Kuo WT; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
  • Lin CP; Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
  • Hou HH; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
Mol Oral Microbiol ; 39(5): 344-353, 2024 Oct.
Article en En | MEDLINE | ID: mdl-38385832
ABSTRACT

INTRODUCTION:

The worldwide prevalence of periodontitis is considerably high, and its pathogenic mechanisms must be investigated and understood in order to improve clinical treatment outcomes and reduce the disease prevalence and burden. The exacerbation of the host immune system induced by oral microbial dysbiosis and the subsequent tissue destruction are the hallmarks of the periodontitis. However, the oral bacteria involved in periodontitis are not fully understood. We used the Oxford Nanopore Technologies (ONT) sequencing system to analyze metagenomic information in subgingival dental plaque from periodontitis and non-periodontitis patients. The number of Lactobacillus zeae (L. zeae) in the periodontitis patients was 17.55-fold higher than in the non-periodontitis patients, suggesting that L. zeae is a novel periodontitis-associated pathogen. Although several Lactobacillus species are used in vivo as probiotics to treat periodontitis and compete with Porphyromonas gingivalis (P. gingivalis), the roles of L. zeae in periodontitis progression, and the relationship between L. zeae and P. gingivalis needs to be investigated.

METHODS:

Both L. zeae and P. gingivalis were inoculated in the ligature-implant site of periodontitis mice. We collected mouse gingival crevicular fluid to analyze inflammatory cytokine secretion using a multiplex assay. Intact or sliced mouse maxilla tissue was used for micro-computed tomography analysis or hematoxylin and eosin staining, immunohistochemistry, and tartrate-resistant acid phosphatase staining to evaluate alveolar bone loss, neutrophil infiltration, and osteoclast activation, respectively.

RESULTS:

We observed that L. zeae competed with P. gingivalis, and it increased inflammatory cytokine secretion at the ligature-implant site. Similar to P. gingivalis, L. zeae promoted ligature-induced neutrophile infiltration, osteoclast activation, and alveolar bone loss.

DISCUSSION:

We, therefore, concluded that L. zeae accelerated the progression of periodontitis in the ligature-induced periodontitis mouse model.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / Porphyromonas gingivalis / Modelos Animales de Enfermedad / Lactobacillus Límite: Animals / Female / Humans / Male Idioma: En Revista: Mol Oral Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Dinamarca
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / Porphyromonas gingivalis / Modelos Animales de Enfermedad / Lactobacillus Límite: Animals / Female / Humans / Male Idioma: En Revista: Mol Oral Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Dinamarca