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Phenotypic virulence characterization of avian pathogenic Escherichia coli (APEC) isolates from broiler breeders with colibacillosis in Mississippi.
Joseph, Jiddu; Magee, Christopher; Jia, Linan; Zhang, Li; Adhikari, Pratima; Ramachandran, Reshma.
Afiliación
  • Joseph J; Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, United States.
  • Magee C; USDA-ARS, Poultry Research Unit, Mississippi State, MS 39762, United States.
  • Jia L; Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, United States.
  • Zhang L; Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, United States.
  • Adhikari P; Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, United States.
  • Ramachandran R; Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, United States.
J Appl Microbiol ; 135(5)2024 May 01.
Article en En | MEDLINE | ID: mdl-38383817
ABSTRACT

AIMS:

In this study, we evaluated the phenotypic virulence characteristics of avian pathogenic Escherichia coli (APEC) isolates from broiler breeders with colibacillosis in Mississippi. Also, the relationship between phenotypic and genotypic virulence patterns was determined. METHODS AND

RESULTS:

Twenty-eight APEC isolated from lesions of broiler breeders diagnosed with colibacillosis were used for embryo lethality assay and chick challenge study. The percentage of embryo mortality following embryo lethality assay and pathogenicity score following the chick challenge study were used to categorize the isolates based on virulence. Pearson correlation analysis was performed to determine the relationship between embryo mortality, chick pathogenicity, and the presence of virulence-associated genes in the isolates. Overall, 39.3% of the isolates were highly virulent and 3.5% were avirulent, following both assays. There existed a positive correlation between embryo mortality and chick pathogenicity (r = 0.73, P < .01), as well as percentage embryo mortality and pathogenicity score with the presence of some virulence genes.

CONCLUSIONS:

Even though all the APEC were isolated from lesions of diseased breeders, the virulence potential varied from being avirulent to highly virulent. Further, we identified a positive relationship between phenotypic virulence and the frequency of virulence-associated genes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Enfermedades de las Aves de Corral / Pollos / Escherichia coli / Infecciones por Escherichia coli Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Enfermedades de las Aves de Corral / Pollos / Escherichia coli / Infecciones por Escherichia coli Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido