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Ultrastructural features of tumor-associated mast cells in parasympathetic paragangliomas (chemodectomas) of the neck.
Chekmaryova, Irina; Kalinin, Dmitry; Kostin, Andrey; Buchwalow, Igor; Tiemann, Markus; Elieh-Ali-Komi, Daniel; Atiakshin, Dmitrii.
Afiliación
  • Chekmaryova I; Federal State Budgetary Institution "National Medical Research Center of Surgery named after A. Vishnevsky", Ministry of Health of the Russian Federation, Moscow, Russia.
  • Kalinin D; Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples' Friendship University of Russia, Moscow, Russia.
  • Kostin A; Federal State Budgetary Institution "National Medical Research Center of Surgery named after A. Vishnevsky", Ministry of Health of the Russian Federation, Moscow, Russia.
  • Buchwalow I; Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples' Friendship University of Russia, Moscow, Russia.
  • Tiemann M; Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples' Friendship University of Russia, Moscow, Russia.
  • Elieh-Ali-Komi D; Institute for Hematopathology, Hamburg, Germany.
  • Atiakshin D; Institute for Hematopathology, Hamburg, Germany.
Microsc Res Tech ; 87(6): 1373-1383, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38380731
ABSTRACT
The mechanisms of the pathogenesis of neck paraganglioma (PGL) and the possible role of mast cells (MCs) in its development and metastasis are still poorly understood. We analyzed MCs' morphologic characterization, activation, and the properties of their cytoplasmic/released granules in PGLs, using light and transmission electron microscopy. Paragangliomas showed a large tumor-associated MC population both in the connective tissue layers of the tumor and between the tumor cells. Notably, MCs were presented by a high expression of specific proteases, size variation, polymorphism, and variable ultrastructural phenotype of granules. A massive number of granules were released surrounding the degranulated MCs while the integrity of MC membrane was maintained. Granules were electron-dense with or without a membrane, ranging from 0.2 to 0.8 µm in diameter. MC plasmalemma was not found at the site of MC-collagen fibrils contact, whereas the secretome and fibrils were directly contacted. We observed direct and mediator-based interactions between MCs and paraganglioma cells. The latter preserved their membrane integrity when MC granules were not in proximity. The effects of the MC secretome on the paraganglioma microenvironment demonstrated its pathogenetic role in tumor progression and allow its application to new diagnostic criteria and the development of protocols for personalized therapy. RESEARCH HIGHLIGHTS Ultrastructural analysis reveals novel regulatory effects of mast cells via diverse secretory pathways on the pathogenesis of parasympathetic paraganglioma, including fibrous extracellular matrix remodeling and mediator-based interactions between MCs and cells of the tumor microenvironment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paraganglioma Extraadrenal / Mastocitos Límite: Humans Idioma: En Revista: Microsc Res Tech Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paraganglioma Extraadrenal / Mastocitos Límite: Humans Idioma: En Revista: Microsc Res Tech Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos