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Cotyledon-Specific Flow Evaluation of Rhesus Macaque Placental Injury Using Ferumoxytol Dynamic Contrast-Enhanced MRI.
Chen, Ruiming; Seiter, Daniel; Keding, Logan T; Vazquez, Jessica; Antony, Kathleen M; Simmons, Heather A; Basu, Puja; Mejia, Andres F; Johnson, Kevin M; Stanic, Aleksandar K; Liu, Ruo-Yu; Shah, Dinesh M; Golos, Thaddeus G; Wieben, Oliver.
Afiliación
  • Chen R; Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Seiter D; Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Keding LT; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Vazquez J; Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Antony KM; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Simmons HA; Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Basu P; Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Mejia AF; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Johnson KM; Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Stanic AK; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Liu RY; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Shah DM; Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Golos TG; Radiology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Wieben O; Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
J Magn Reson Imaging ; 60(5): 2196-2204, 2024 Nov.
Article en En | MEDLINE | ID: mdl-38375996
ABSTRACT

BACKGROUND:

Recently, dynamic contrast-enhanced (DCE) MRI with ferumoxytol as contrast agent has recently been introduced for the noninvasive assessment of placental structure and function throughout. However, it has not been demonstrated under pathological conditions.

PURPOSE:

To measure cotyledon-specific rhesus macaque maternal placental blood flow using ferumoxytol DCE MRI in a novel animal model for local placental injury. STUDY TYPE Prospective animal model.

SUBJECTS:

Placental injections of Tisseel (three with 0.5 mL and two with 1.5 mL), monocyte chemoattractant protein 1 (three with 100 µg), and three with saline as controls were performed in a total of 11 rhesus macaque pregnancies at approximate gestational day (GD 101). DCE MRI scans were performed prior (GD 100) and after (GD 115 and GD 145) the injection (term = GD 165). FIELD STRENGTH/SEQUENCE 3 T, T1-weighted spoiled gradient echo sequence (product sequence, DISCO). ASSESSMENT Source images were inspected for motion artefacts from the mother or fetus. Placenta segmentation and DCE processing were performed for the dynamic image series to measure cotyledon specific volume, flow, and normalized flow. Overall placental histopathology was conducted for controls, Tisseel, and MCP-1 animals and regions of tissue infarctions and necrosis were documented. Visual inspections for potential necrotic tissue were conducted for the two Tisseelx3 animals. STATISTICAL TESTS Wilcoxon rank sum test, significance level P < 0.05.

RESULTS:

No motion artefacts were observed. For the group treated with 1.5 mL of Tisseel, significantly lower cotyledon volume, flow, and normalized flow per cotyledon were observed for the third gestational time point of imaging (day ~145), with mean normalized flow of 0.53 minute-1. Preliminary histopathological analysis shows areas of tissue necrosis from a selected cotyledon in one Tisseel-treated (single dose) animal and both Tisseelx3 (triple dose) animals. DATA

CONCLUSION:

This study demonstrates the feasibility of cotyledon-specific functional analysis at multiple gestational time points and injury detection in a placental rhesus macaque model through ferumoxytol-enhanced DCE MRI. LEVEL OF EVIDENCE NA TECHNICAL EFFICACY Stage 2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Imagen por Resonancia Magnética / Medios de Contraste / Óxido Ferrosoférrico / Macaca mulatta Límite: Animals / Pregnancy Idioma: En Revista: J Magn Reson Imaging Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Imagen por Resonancia Magnética / Medios de Contraste / Óxido Ferrosoférrico / Macaca mulatta Límite: Animals / Pregnancy Idioma: En Revista: J Magn Reson Imaging Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos