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Molecular characterization of Haemoproteus enucleator with emphasis on the host and geographic distribution.
Tchoumbou, Mélanie; Harl, Josef; Souaibou, Aladji; Iezhova, Tatjana; Valkiunas, Gediminas.
Afiliación
  • Tchoumbou M; Nature Research Centre, Akademijos 2, 08412 Vilnius, Lithuania; Biodiversity Initiative, Houghton, MI, USA. Electronic address: tchoumbou4@gmail.com.
  • Harl J; Institute of Pathology, Department for Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
  • Souaibou A; Vector Borne Parasitic & Infectious Diseases Laboratory of the Applied Biology and Ecology Research Unit, University of Dschang, Cameroon.
  • Iezhova T; Nature Research Centre, Akademijos 2, 08412 Vilnius, Lithuania.
  • Valkiunas G; Nature Research Centre, Akademijos 2, 08412 Vilnius, Lithuania.
Acta Trop ; 253: 107154, 2024 May.
Article en En | MEDLINE | ID: mdl-38373526
ABSTRACT
Haemoproteus species (Haemosporida, Haemoproteidae) are cosmopolitan and highly diverse blood parasites of birds that have been neglected in avian medicine. However, recent discoveries based on molecular diagnostic markers show that these pathogens often cause marked damage to various internal organs due to exo-erythrocytic development, sometimes resulting in severe and even lethal avian haemoproteosis, including cerebral pathologies. Molecular markers are essential for haemoproteosis diagnostics, but the data is limited, particularly for parasites transmitted in tropical ecosystems. This study combined microscopic and molecular approaches to characterize Haemoproteus enucleator morphologically and molecularly. Blood samples were collected from the African pygmy kingfisher Ispidina picta in Cameroon, and the parasite was identified using morphological characters of gametocytes. The analysis of partial cytochrome b sequences (cytb) identified a new Haemoproteus lineage (hISPIC03), which was linked to the morphospecies H. enucleator. Illustrations of blood stages were provided and the phylogenetic analysis showed that the new lineage clustered with five other closely related lineages belonging to the same morphospecies (hALCLEU01, hALCLEU02, hALCLEU03, hISPIC01, and hALCQUA01), with a maximum genetic distance between these lineages of 1.5 % (7 bp difference) in the 478 bp cytb sequences. DNA haplotype network was developed and identified geographic and host distribution of all lineages belonging to H. enucleator group. These lineages were almost exclusively detected in African kingfishers from Gabon, Cameroon, South Africa, and Botswana. This study developed the molecular characterization of H. enucleator and provides opportunities for diagnostics of this pathogen at all stages of its life cycle, which remains undescribed in all its closely related lineages.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Protozoarias en Animales / Enfermedades de las Aves / Haemosporida Límite: Animals Idioma: En Revista: Acta Trop Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Protozoarias en Animales / Enfermedades de las Aves / Haemosporida Límite: Animals Idioma: En Revista: Acta Trop Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos