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DNA nanostructure decoration: a how-to tutorial.
Piantanida, Luca; Liddle, J Alexander; Hughes, William L; Majikes, Jacob M.
Afiliación
  • Piantanida L; Faculty of Applied Science, School of Engineering, University of British Columbia, Kelowna, B.C., V1V 1V7, Canada.
  • Liddle JA; National Institute of Standards and Technology, Gaithersburg, MD, 20878, United States of America.
  • Hughes WL; Faculty of Applied Science, School of Engineering, University of British Columbia, Kelowna, B.C., V1V 1V7, Canada.
  • Majikes JM; National Institute of Standards and Technology, Gaithersburg, MD, 20878, United States of America.
Nanotechnology ; 35(27)2024 Apr 23.
Article en En | MEDLINE | ID: mdl-38373400
ABSTRACT
DNA Nanotechnology is being applied to multiple research fields. The functionality of DNA nanostructures is significantly enhanced by decorating them with nanoscale moieties including proteins, metallic nanoparticles, quantum dots, and chromophores. Decoration is a complex process and developing protocols for reliable attachment routinely requires extensive trial and error. Additionally, the granular nature of scientific communication makes it difficult to discern general principles in DNA nanostructure decoration. This tutorial is a guidebook designed to minimize experimental bottlenecks and avoid dead-ends for those wishing to decorate DNA nanostructures. We supplement the reference material on available technical tools and procedures with a conceptual framework required to make efficient and effective decisions in the lab. Together these resources should aid both the novice and the expert to develop and execute a rapid, reliable decoration protocols.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Nanotecnología / Nanoestructuras Idioma: En Revista: Nanotechnology Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Nanotecnología / Nanoestructuras Idioma: En Revista: Nanotechnology Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido