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Novel Sheep Model to Assess Critical-Sized Bone Regeneration with Periosteum for In Vivo Bioreactors.
Ghosh, Yohaann A; Xin, Hai; Al Maruf, D S Abdullah; Cheng, Kai; Wise, Innes; Burrows, Chris; Gupta, Ruta; Cheung, Veronica Ka-Yan; Wykes, James; Leinkram, David; Froggatt, Catriona; Lewin, Will; Kruse, Hedi V; Tomaskovic-Crook, Eva; McKenzie, David R; Crook, Jeremy; Clark, Jonathan R.
Afiliación
  • Ghosh YA; Department of Head & Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia.
  • Xin H; Sydney Medical School, The University of Sydney, Camperdown, Australia.
  • Al Maruf DSA; School of Medicine & Dentistry, Griffith University, Gold Coast, Australia.
  • Cheng K; Department of Head & Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia.
  • Wise I; Sydney Medical School, The University of Sydney, Camperdown, Australia.
  • Burrows C; Department of Head & Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia.
  • Gupta R; Sydney Medical School, The University of Sydney, Camperdown, Australia.
  • Cheung VK; Department of Head & Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia.
  • Wykes J; Royal Prince Alfred Institute of Academic Surgery, Sydney Local Health District, Australia.
  • Leinkram D; Laboratory Animal Services, The University of Sydney, Camperdown, Australia.
  • Froggatt C; Laboratory Animal Services, The University of Sydney, Camperdown, Australia.
  • Lewin W; Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Camperdown, Australia.
  • Kruse HV; Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Camperdown, Australia.
  • Tomaskovic-Crook E; Department of Head & Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia.
  • McKenzie DR; Sydney Medical School, The University of Sydney, Camperdown, Australia.
  • Crook J; Department of Head & Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia.
  • Clark JR; Department of Head & Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia.
Tissue Eng Part C Methods ; 30(4): 159-169, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38368556
ABSTRACT
Considerable research is being undertaken to develop novel biomaterials-based approaches for surgical reconstruction of bone defects. This extends to three-dimensional (3D) printed materials that provide stable, structural, and functional support in vivo. However, few preclinical models can simulate in vivo human biological conditions for clinically relevant testing. In this study we describe a novel ovine model that allows evaluation of in vivo osteogenesis via contact with bone and/or periosteum interfaced with printed polymer bioreactors loaded with biomaterial bone substitutes. The infraspinous scapular region of 14 Dorset cross sheep was exposed. Vascularized periosteum was elevated either attached to the infraspinatus muscle or separately. In both cases, the periosteum was supplied by the periosteal branch of the circumflex scapular vessels. In eight sheep, a 3D printed 4-chambered polyetheretherketone bioreactor was wrapped circumferentially in vascularized periosteum. In 6 sheep, 12 double-sided 3D printed 2-chambered polyetherketone bioreactors were secured to the underlying bone allowing direct contact with the bone on one side and periosteum on the other. Our model enabled simultaneous testing of up to 24 (12 double-sided) 10 × 10 × 5 mm bioreactors per scapula in the flat contact approach or a single 40 × 10 mm four-chambered bioreactor per scapula using the periosteal wrap. De novo bone growth was evaluated using histological and radiological analysis. Of importance, the experimental model was well tolerated by the animals and provides a versatile approach for comparing the osteogenic potential of cambium on the bone surface and elevated with periosteum. Furthermore, the periosteal flaps were sufficiently large for encasing bioreactors containing biomaterial bone substitutes for applications such as segmental mandibular reconstruction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periostio / Sustitutos de Huesos Límite: Animals / Humans Idioma: En Revista: Tissue Eng Part C Methods Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periostio / Sustitutos de Huesos Límite: Animals / Humans Idioma: En Revista: Tissue Eng Part C Methods Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos