Your browser doesn't support javascript.
loading
Cellular uptake and in vivo distribution of mesenchymal-stem-cell-derived extracellular vesicles are protein corona dependent.
Liam-Or, Revadee; Faruqu, Farid N; Walters, Adam; Han, Shunping; Xu, Lizhou; Wang, Julie Tzu-Wen; Oberlaender, Jennifer; Sanchez-Fueyo, Alberto; Lombardi, Giovanna; Dazzi, Francesco; Mailaender, Volker; Al-Jamal, Khuloud T.
Afiliación
  • Liam-Or R; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, London, UK.
  • Faruqu FN; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, London, UK.
  • Walters A; Pharmacology Department, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
  • Han S; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, London, UK.
  • Xu L; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, London, UK.
  • Wang JT; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, London, UK.
  • Oberlaender J; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, London, UK.
  • Sanchez-Fueyo A; Max Planck Institute for Polymer Research, Mainz, Germany.
  • Lombardi G; Department of Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
  • Dazzi F; Institute of Liver Studies, King's College London University and King's College Hospital, London, UK.
  • Mailaender V; Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, UK.
  • Al-Jamal KT; Comprehensive Cancer Centre, King's College London, London, UK.
Nat Nanotechnol ; 19(6): 846-855, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38366223
ABSTRACT
Extracellular vesicles (EVs) derived from mesenchymal stem cells are promising nanotherapeutics in liver diseases due to their regenerative and immunomodulatory properties. Nevertheless, a concern has been raised regarding the rapid clearance of exogenous EVs by phagocytic cells. Here we explore the impact of protein corona on EVs derived from two culturing conditions in which specific proteins acquired from media were simultaneously adsorbed on the EV surface. Additionally, by incubating EVs with serum, simulating protein corona formation upon systemic delivery, further resolved protein corona-EV complex patterns were investigated. Our findings reveal the potential influences of corona composition on EVs under in vitro conditions and their in vivo kinetics. Our data suggest that bound albumin creates an EV signature that can retarget EVs from hepatic macrophages. This results in markedly improved cellular uptake by hepatocytes, liver sinusoidal endothelial cells and hepatic stellate cells. This phenomenon can be applied as a camouflage strategy by precoating EVs with albumin to fabricate the albumin-enriched protein corona-EV complex, enhancing non-phagocytic uptake in the liver. This work addresses a critical challenge facing intravenously administered EVs for liver therapy by tailoring the protein corona-EV complex for liver cell targeting and immune evasion.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas / Vesículas Extracelulares / Corona de Proteínas Límite: Animals / Humans Idioma: En Revista: Nat Nanotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas / Vesículas Extracelulares / Corona de Proteínas Límite: Animals / Humans Idioma: En Revista: Nat Nanotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido