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Chromatograms and Mass Spectra of High-Mannose and Paucimannose N-Glycans for Rapid Isomeric Identifications.
Liew, Chia Yen; Chen, Jien-Lian; Lin, Yen-Ting; Luo, Hong-Sheng; Hung, An-Ti; Magoling, Bryan John Abel; Nguan, Hock-Seng; Lai, Charles Pin-Kuang; Ni, Chi-Kung.
Afiliación
  • Liew CY; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106216, Taiwan.
  • Chen JL; International Graduate Program of Molecular Science and Technology, National Taiwan University, Taipei 106216, Taiwan.
  • Lin YT; Molecular Science and Technology, Taiwan International Graduate Program, Academia Sinica, Taipei 106216, Taiwan.
  • Luo HS; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106216, Taiwan.
  • Hung AT; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106216, Taiwan.
  • Magoling BJA; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106216, Taiwan.
  • Nguan HS; Department of Chemistry, National Taiwan Normal University, Taipei 116059, Taiwan.
  • Lai CP; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106216, Taiwan.
  • Ni CK; Department of Chemistry, National Tsing Hua University, Hsinchu 300044, Taiwan.
J Proteome Res ; 23(3): 939-955, 2024 03 01.
Article en En | MEDLINE | ID: mdl-38364797
ABSTRACT
N-Linked glycosylation is one of the most essential post-translational modifications of proteins. However, N-glycan structural determination remains challenging because of the small differences in structures between isomers. In this study, we constructed a database containing collision-induced dissociation MSn mass spectra and chromatograms of high-performance liquid chromatography for the rapid identification of high-mannose and paucimannose N-glycan isomers. These N-glycans include isomers by breaking of arbitrary numbers of glycosidic bonds at arbitrary positions of canonical Man9GlcNAc2 N-glycans. In addition, some GlcMannGlcNAc2 N-glycan isomers were included in the database. This database is particularly useful for the identification of the N-glycans not in conventional N-glycan standards. This study demonstrated the application of the database to structural assignment for high-mannose N-glycans extracted from bovine whey proteins, soybean proteins, human mammary epithelial cells, and human breast carcinoma cells. We found many N-glycans that are not expected to be generated by conventional biosynthetic pathways of multicellular eukaryotes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mama / Manosa Límite: Animals / Humans Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mama / Manosa Límite: Animals / Humans Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos