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Methionine sources and genotype affect embryonic intestinal development, antioxidants, tight junctions, and growth-related gene expression in chickens.
Lugata, James K; Ndunguru, Sawadi F; Reda, Gebrehaweria K; Ozsváth, Xénia E; Angyal, Eszter; Czeglédi, Levente; Gulyás, Gabriella; Knop, Renáta; Oláh, János; Mészár, Zoltán; Varga, Rita; Csernus, Brigitta; Szabó, Csaba.
Afiliación
  • Lugata JK; Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
  • Ndunguru SF; Faculty of Agriculture and Food Sciences and Environmental Management, Doctoral School of Animal Science, University of Debrecen, Debrecen, Hungary.
  • Reda GK; Department of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
  • Ozsváth XE; Faculty of Agriculture and Food Sciences and Environmental Management, Doctoral School of Animal Science, University of Debrecen, Debrecen, Hungary.
  • Angyal E; Department of Evolutionary Zoology and Human Biology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
  • Czeglédi L; Department of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
  • Gulyás G; Faculty of Agriculture and Food Sciences and Environmental Management, Doctoral School of Animal Science, University of Debrecen, Debrecen, Hungary.
  • Knop R; Department of Evolutionary Zoology and Human Biology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
  • Oláh J; Department of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
  • Mészár Z; Department of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
  • Varga R; Department of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
  • Csernus B; Department of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
  • Szabó C; Department of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
Anim Nutr ; 16: 218-230, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38362512
ABSTRACT
Methionine (Met) is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and growth. The present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and health. Fertilized eggs of the two genotypes, TETRA-SL layer hybrid (TSL) - commercial layer hybrid and Hungarian Partridge colored hen breed (HPC) - a native genotype, were randomly distributed into four treatments for each genotype. The treatment groups include the following 1) control non-injected eggs (NoIn); 2) saline-injected (SaIn); 3) DL-Met injected (DLM); and 4) L-Met injected (LM). The in ovo injection was carried out on 17.5 d of embryonic development; after hatching, eight chicks per group were sacrificed, and the jejunum was extracted for analysis. The results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width, villus height, and villus area (P < 0.05) compared to the control. The DLM group had significantly reduced crypt depth, glutathione content (GSH), glutathione S-transferase 3 alpha (GST3), occludin (OCLN) gene expression and increased villus height to crypt depth ratio in the TSL genotype than the LM group (P < 0.05). The HPC genotype has overexpressed insulin-like growth factor 1 (IGF1) gene, tricellulin (MD2), occludin (OCLN), superoxide dismutase 1 (SOD1), and GST3 genes than the TSL genotype (P < 0.05). In conclusion, these findings showed that in ovo injection of Met enhanced intestinal development, and function, with genotypes responding differently under normal conditions. Genotypes also influenced the expression of intestinal antioxidants, tight junction, and growth-related genes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anim Nutr Año: 2024 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anim Nutr Año: 2024 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: China