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Development of a "Rapid-Crypto Colorimetric LAMP Test" to Detect Cryptosporidiosis in Feces of Newborns Calves.
Karakavuk, Muhammet; Can, Hüseyin; Can, Sengül; Karakavuk, Tugba; Döskaya, Mert; Degirmenci Döskaya, Aysu.
Afiliación
  • Karakavuk M; Ege University, Ödemis Vocational School, Ödemis, Izmir, Türkiye.
  • Can H; Ucyildiz Biotechnology and Veterinary Services, Yunusemre, Manisa, Türkiye.
  • Can S; Ege University, Vaccine Development Application and Research Center, Bornova, Izmir, Türkiye.
  • Karakavuk T; Ege University, Vaccine Development Application and Research Center, Bornova, Izmir, Türkiye.
  • Döskaya M; Ege University, Faculty of Science, Department of Biology Molecular Biology Section, Bornova, Izmir, Türkiye.
  • Degirmenci Döskaya A; Ucyildiz Biotechnology and Veterinary Services, Yunusemre, Manisa, Türkiye.
Acta Parasitol ; 69(1): 691-699, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38358452
ABSTRACT

BACKGROUND:

Cryptosporidiosis is a disease that causes major intestinal damage in humans and animals. The causative agents of the disease are Cryptosporidium species. In newborn calves, diarrhea can lead to death, resulting in significant economic losses for the farms. Therefore, accurate, rapid, and cost-effective diagnosis of the disease is very important. MATERIAL AND

METHODS:

In this study, a novel colorimetric loop-mediated isothermal amplification (LAMP) test named "Rapid-Crypto Colorimetric LAMP test" targeting Cryptosporidium spp. 18S rRNA gene was developed to detect cryptosporidiosis in the feces of newborn calves. The analytical sensitivity of the test was determined by plasmid controls. Clinical sensitivity was determined using the feces of 127 calves collected from farms in Izmir and Manisa provinces. All of the samples were also investigated with Real-Time PCR targeting the Cryptosporidium spp. COWP gene. Cross-reactivity was tested using the DNA of other parasites and bacteria.

RESULTS:

According to the results, the analytical sensitivity of the "Rapid-Crypto Colorimetric LAMP test" was found as 1 copy plasmid/reaction. When the results were compared with the Real-Time PCR test, the sensitivity of the "Rapid-Crypto Colorimetric LAMP test" was 100% and the specificity was 97.4%. The test did not cross-react with other parasites and bacteria.  

CONCLUSION:

The "Rapid-Crypto Colorimetric LAMP test" developed in this study provides an advantage in the diagnosis of Cryptosporidium spp. in calf stool samples since it can be applied in basic laboratories or in the field, does not require experienced personnel, and has high sensitivity. Moreover, diagnosis can be made with the naked eye without using any device.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Bovinos / Sensibilidad y Especificidad / Colorimetría / Criptosporidiosis / Cryptosporidium / Técnicas de Amplificación de Ácido Nucleico / Heces / Animales Recién Nacidos Límite: Animals Idioma: En Revista: Acta Parasitol Año: 2024 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Bovinos / Sensibilidad y Especificidad / Colorimetría / Criptosporidiosis / Cryptosporidium / Técnicas de Amplificación de Ácido Nucleico / Heces / Animales Recién Nacidos Límite: Animals Idioma: En Revista: Acta Parasitol Año: 2024 Tipo del documento: Article Pais de publicación: Suiza