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Usefulness of a new immunochromatographic assay using fluorescent silica nanoparticles for serodiagnosis of Thai patients with amebiasis.
Kakino, Azumi; Pattanawong, Urassaya; Kuamsab, Napaporn; Imai, Tatsuya; Putaporntip, Chaturong; Asai, Satomi; Cheng, Xunjia; Jongwutiwes, Somchai; Tachibana, Hiroshi.
Afiliación
  • Kakino A; Department of Parasitology, Tokai University School of Medicine, Isehara, Japan.
  • Pattanawong U; Department of Parasitology, Tokai University School of Medicine, Isehara, Japan.
  • Kuamsab N; Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Imai T; Department of Parasitology, Tokai University School of Medicine, Isehara, Japan.
  • Putaporntip C; Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Asai S; Community Public Health Program, Faculty of Health Science and Technology, Southern College of Technology, Nakorn Si Thammarat, Thailand.
  • Cheng X; Department of Parasitology, Tokai University School of Medicine, Isehara, Japan.
  • Jongwutiwes S; Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Tachibana H; Department of Laboratory Medicine, Tokai University School of Medicine, Isehara, Japan.
Drug Discov Ther ; 18(1): 10-15, 2024 Mar 20.
Article en En | MEDLINE | ID: mdl-38355121
ABSTRACT
A fluorescence immunochromatography (FIC) kit was developed recently using fluorescent silica nanoparticles coated with a recombinant C-terminal fragment of the surface lectin intermediate subunit (C-Igl) of Entamoeba histolytica to establish rapid serodiagnosis of amebiasis. We further evaluated the system using serum samples from 52 Thai patients with amebiasis. Of the patients, 50 (96%) tested positive using FIC. The samples were also tested using enzyme-linked immunosorbent assay (ELISA) with C-Igl as the antigen. Two samples were negative on ELISA but positive on FIC. The correlation coefficient between the fluorescence intensity using FIC and the optical density value using ELISA was 0.5390, indicating a moderate correlation between the two tests. Serum samples from 20 patients with malaria and 22 patients with Clostridioides difficile infection were also tested using FIC. The false-positive rates were 4/20 (20%) and 1/22 (4%) in patients with malaria and C. difficile infection, respectively. Combining the data from the present study with our previous study, the sensitivity and specificity of FIC were determined to be 98.5% and 95.2%, respectively. The results of the 50 samples were studied using a fluorescence scope and a fluorescence intensity reader, and the findings were compared. Disagreements were found in only two samples showing near-borderline fluorescence intensity, indicating that the use of scope was adequate for judging the results. These results demonstrate that FIC is a simple and rapid test for the serodiagnosis of amebiasis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clostridioides difficile / Entamebiasis / Nanopartículas / Amebiasis / Malaria Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Drug Discov Ther Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clostridioides difficile / Entamebiasis / Nanopartículas / Amebiasis / Malaria Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Drug Discov Ther Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón