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[Development of selective method for mebeverine detection in blood]. / Razrabotka selektivnoi metodiki opredeleniya mebeverina v krovi.
Vikman, P S; Balabanova, O L; Strelova, O Yu; Grebenyuk, A N.
Afiliación
  • Vikman PS; Saint Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.
  • Balabanova OL; Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine, St. Petersburg, Russia.
  • Strelova OY; Saint Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.
  • Grebenyuk AN; Saint Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.
Sud Med Ekspert ; 67(1): 34-39, 2024.
Article en Ru | MEDLINE | ID: mdl-38353013
ABSTRACT

OBJECTIVE:

To develop the method of biological fluids' sample processing and mebeverine detection to exclude false results' receiving when diagnosing drug intoxication. MATERIAL AND

METHODS:

The study was carried out using «Mebeverine¼ (NJCO «North star¼, Russia) medicine and hydrolysis by enzymes, namely papain, chymotrypsin, trypsin, chymopsin and hyaluronidase, was applied for sample processing. The extractions were analyzed by methods of HPLC-MS/MS on Nexera XR modular liquid chromatograph with LCMS-8050 (Shimadzu) tandem mass spectrometer and GC-MS on gas chromatograph connected with QP-2020 (Shimadzu, Japanese) mono quadrupole mass spectrometer. RESULTS AND

CONCLUSION:

It has been revealed that using selective method of sample processing, which consists of aqueous phase extraction at pH=2-4 and enzymatic hydrolysis by papain and hyaluronidase during isolation from blood, is required to detect mebeverine in biological liquids. It has been proven that the native mebeverine is hydrolyzed to veratric (3.4-dimethoxybenzoic) acid and mebeverine alcohol at alkalotic pH value of medium. It has been shown that mebeverine extraction is necessary to study using HPLC-MS/MS, which will allow to avoid the native mebeverine degradation in chromatograph injector as with GC-MS method analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenetilaminas / Papaína / Espectrometría de Masas en Tándem Tipo de estudio: Diagnostic_studies Idioma: Ru Revista: Sud Med Ekspert Año: 2024 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Rusia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenetilaminas / Papaína / Espectrometría de Masas en Tándem Tipo de estudio: Diagnostic_studies Idioma: Ru Revista: Sud Med Ekspert Año: 2024 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Rusia