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Temperature impacts the bovine ex vivo immune response towards Mycoplasmopsis bovis.
Démoulins, Thomas; Yimthin, Thatcha; Lindtke, Dorothea; Eggerschwiler, Lukas; Siegenthaler, Raphael; Labroussaa, Fabien; Jores, Joerg.
Afiliación
  • Démoulins T; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland. thomas.demoulins@unibe.ch.
  • Yimthin T; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland.
  • Lindtke D; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
  • Eggerschwiler L; Animal GenoPhenomics Group, Agroscope, 1725, Posieux, Switzerland.
  • Siegenthaler R; Research Contracts Animals Group, Agroscope, 1725, Posieux, Switzerland.
  • Labroussaa F; Research Contracts Animals Group, Agroscope, 1725, Posieux, Switzerland.
  • Jores J; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland.
Vet Res ; 55(1): 18, 2024 Feb 13.
Article en En | MEDLINE | ID: mdl-38351086
ABSTRACT
Although cattle are the mammalian species with most global biomass associated with a huge impact on our planet, their immune system remains poorly understood. Notably, the bovine immune system has peculiarities such as an overrepresentation of γδ T cells that requires particular attention, specifically in an infectious context. In line of 3R principles, we developed an ex vivo platform to dissect host-pathogen interactions. The experimental design was based on two independent complementary readouts firstly, a novel 12-14 color multiparameter flow cytometry assay measuring maturation (modulation of cell surface marker expression) and activation (intracellular cytokine detection) of monocytes, conventional and plasmacytoid dendritic cells, natural killer cells, γδ T cells, B and T cells; secondly, a multiplex immunoassay monitoring bovine chemokine and cytokine secretion levels. The experiments were conducted on fresh primary bovine blood cells exposed to Mycoplasmopsis bovis (M. bovis), a major bovine respiratory pathogen. Besides reaffirming the tight cooperation of the different primary blood cells, we also identified novel key players such as strong IFN-γ secreting NK cells, whose role was so far largely overlooked. Additionally, we compared the host-pathogen interactions at different temperatures, including commonly used 37 °C, ruminant body temperature (38-38.5 °C) and fever (≥ 39.5 °C). Strikingly, working under ruminant physiological temperature influenced the capacity of most immune cell subsets to respond to M. bovis compared to 37 °C. Under fever-like temperature conditions the immune response was impaired compared to physiological temperature. Our experimental approach, phenotypically delineating the bovine immune system provided a thorough vision of the immune response towards M. bovis and the influence of temperature towards that immune response.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Bovina / Enfermedades de los Bovinos / Mycobacterium bovis Límite: Animals Idioma: En Revista: Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Bovina / Enfermedades de los Bovinos / Mycobacterium bovis Límite: Animals Idioma: En Revista: Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido