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A 19-year longitudinal study to characterize carbapenem-nonsusceptible Acinetobacter isolated from patients with bloodstream infections and the contribution of conjugative plasmids to carbapenem resistance and virulence.
Chen, Pek Kee; Lee, Yi-Tzu; Liu, Chia-Ying; Thuy, Tran Thi Dieu; Anh, Kieu; Wu, Jiunn-Jong; Liao, Chun-Hsing; Huang, Yu-Tsung; Chen, Yu-Chen; Kao, Cheng-Yen.
Afiliación
  • Chen PK; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • Lee YT; Department of Emergency Medicine, Taipei Veteran General Hospital, Taipei, Taiwan; School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • Liu CY; Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
  • Thuy TTD; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • Anh K; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • Wu JJ; Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan.
  • Liao CH; Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
  • Huang YT; Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.
  • Chen YC; Genomics Research Center, Academia Sinica, Taipei, Taiwan.
  • Kao CY; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan; Health Innovation Center, National Yang Ming Chiao Tung University, Taiwan; Microbiota Research Center, National Yang Ming Chiao Tung University, Taiwan. Electronic address:
J Microbiol Immunol Infect ; 57(2): 288-299, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38350841
ABSTRACT

BACKGROUND:

This study aimed to characterize carbapenem-nonsusceptible Acinetobacter (CNSA) isolated from patients with bacteremia from 1997 to 2015.

METHODS:

A total of 173 CNSA (12.3%) was recovered from 1403 Acinetobacter isolates. The presence of selected ß-lactamase genes in CNSA was determined by PCR amplification. The conjugation test was used to determine the transferability of metallo-ß-lactamase (MBL)-carrying plasmids. Whole genome sequencing in combination with phenotypic assays was carried out to characterize MBL-plasmids.

RESULTS:

In general, a trend of increasing numbers of CNSA was observed. Among the 173 CNSA, A. baumannii (54.9%) was the most common species, followed by A. nosocomialis (23.1%) and A. soli (12.1%). A total of 49 (28.3%) CNSA were extensively drug-resistant, and all were A. baumannii. The most common class D carbapenemase gene in 173 CNSA was blaOXA-24-like (32.4%), followed by ISAba1-blaOXA-51-like (20.8%), ISAba1-blaOXA-23 (20.2%), and IS1006/IS1008-blaOXA-58 (11.6%). MBL genes, blaVIM-11,blaIMP-1, and blaIMP-19 were detected in 9 (5.2%), 20 (11.6%), and 1 (0.6%) CNSA isolates, respectively. Transfer of MBL genes to AB218 and AN254 recipient cells was successful for 7 and 6 of the 30 MBL-plasmids, respectively. The seven AB218-derived transconjugants carrying MBL-plasmids produced less biofilm but showed higher virulence to larvae than recipient AB218.

CONCLUSIONS:

Our 19-year longitudinal study revealed a stable increase in CNSA during 2005-2015. blaOXA-24-like, ISAba1-blaOXA-51-like, and ISAba1-blaOXA-23 were the major determinants of Acinetobacter carbapenem resistance. MBL-carrying plasmids contribute not only to the carbapenem resistance but also to A. baumannii virulence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sepsis / Acinetobacter baumannii Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: J Microbiol Immunol Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sepsis / Acinetobacter baumannii Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: J Microbiol Immunol Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido