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Implementation of Silver Nanoparticles Green Synthesized with Leaf Extract of Coccinia grandis as Antimicrobial Agents Against Head and Neck Infection MDR Pathogens.
Lenka, Smarita; Dubey, Debasmita; Swain, Santosh Kumar; Rath, Goutam; Mishra, Ajit; Bishoyi, Ajit Kumar; Purohit, Gopal Krishna.
Afiliación
  • Lenka S; Department of Medical Research, IMS and SUM Hospital, Siksha 'O' Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, 751003, Odisha, India.
  • Dubey D; Department of Medical Research, IMS and SUM Hospital, Siksha 'O' Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, 751003, Odisha, India.
  • Swain SK; Department of Otorhinolaryngology and Head and Neck Surgery, All India Institute of Medical Sciences, Sijua, Patrapada, Bhubaneswar, 751019, Odisha, India.
  • Rath G; School of Pharmaceutical Sciences, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, 751003, Odisha, India.
  • Mishra A; School of Pharmaceutical Sciences, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, 751003, Odisha, India.
  • Bishoyi AK; Clinical Hematology, IMS and SUM Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, 751003, Odisha, India.
  • Purohit GK; Heredity Biosciences LLP. Bhubaneswar, 751015, Odisha, India.
Curr Pharm Biotechnol ; 25(17): 2312-2325, 2024.
Article en En | MEDLINE | ID: mdl-38347796
ABSTRACT

BACKGROUND:

Head and neck infections (HNI) associated with multidrug resistance (MDR) offer several health issues on a global scale due to inaccurate diagnosis.

OBJECTIVES:

This study aimed to identify the bacteria and Candidal isolates and implement the silver nanoparticles green synthesized with leaf extract of Coccinia grandis (Cg-AgNPs) as a therapeutic approach against HNI pathogens.

METHODS:

The Cg-AgNPs were characterized by the UV-visible spectrophotometer, FT-IR analysis, Zeta particle size, Zeta potential, and field emission scanning electron microscope (FESEM) analysis to validate the synthesis of nanoparticles. Additionally, the antimicrobial activity of Cg-AgNPs was presented by the zone of inhibition (ZOI), minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration (MBC/MFC), and antibiofilm assay. Moreover, the cell wall rupture assay was visualized on SEM for the morphological study of antimicrobial activities, and the in-vivo toxicity was performed in a swiss mice model to evaluate the impact of Cg-AgNPs on various biological parameters.

RESULTS:

Different bacterial strains (Staphylococcus aureus, Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa) and Candida sp. (Candida albicans, Candida tropicalis, Candida orthopsilosis, and Candida glabrata) were identified. The MIC, MBC, and antibiofilm potential of Cg-AgNPs were found to be highest against A. baumannii 1.25 µg/ml, 5 µg/ml, and 85.01±5.19% respectively. However, C. albicans and C. orthopsilosis revealed 23 mm and 21 mm of ZOI. Subsequently, the micromorphology of the cell wall rupture assay confirmed the efficacy of Cg-AgNPs, and no significant alterations were seen in biochemical and hematological parameters on the swiss mice model in both acute and subacute toxicity studies.

CONCLUSION:

The green synthesized Cg-AgNPs have multifunctional activities like antibacterial, anticandidal, and antibiofilm activity with no toxicity and can be introduced against the HNI pathogens.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Extractos Vegetales / Pruebas de Sensibilidad Microbiana / Hojas de la Planta / Cucurbitaceae / Nanopartículas del Metal / Tecnología Química Verde Límite: Animals Idioma: En Revista: Curr Pharm Biotechnol Asunto de la revista: BIOTECNOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Extractos Vegetales / Pruebas de Sensibilidad Microbiana / Hojas de la Planta / Cucurbitaceae / Nanopartículas del Metal / Tecnología Química Verde Límite: Animals Idioma: En Revista: Curr Pharm Biotechnol Asunto de la revista: BIOTECNOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos