Quantitation of MUC5AC and MUC5B by Stable Isotope Labeling Mass Spectrometry.

Radicioni, Giorgia; Kesimer, Mehmet

Radicioni, Giorgia; Kesimer, Mehmet.

Afiliación

Radicioni G; Marsico Lung Institute/Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC, USA. radicion@email.unc.edu.
Kesimer M; Marsico Lung Institute/Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC, USA.

Methods Mol Biol ; 2763: 125-136, 2024.

Article en En | MEDLINE | ID: mdl-38347406

ABSTRACT

ABSTRACT

Mucins MUC5AC and MUC5B are large glycoproteins that play an essential role in the innate defense of epithelial surfaces and their quantitation in biological samples would be informative about the health status of the tissue/samples they are derived from. However, they are difficult to study and quantify with traditional methods such as ELISA and western blot, due to their size, heterogeneity, and high degree of glycosylation. We successfully implemented a stable isotope labeling mass spectrometry approach for absolute quantification of mucin macromolecules. Here, in detail, we describe this accurate and sensitive liquid chromatography and mass spectrometry (LC-MS) method applied for both MUC5AC and MUC5B quantification in diverse and complex biological samples.

Asunto(s)

Glicoproteínas; Mucinas; Marcaje Isotópico; Espectrometría de Masas; Ensayo de Inmunoadsorción Enzimática

Palabras clave

MUC5AC; MUC5B; Proteomics; Stable isotope labeling mass spectrometry

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas / Mucinas Aspecto: Patient_preference Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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