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Photosynthetic production of α-farnesene by engineered Synechococcus elongatus UTEX 2973 from carbon dioxide.
Rautela, Akhil; Yadav, Indrajeet; Gangwar, Agendra; Chatterjee, Rishika; Kumar, Sanjay.
Afiliación
  • Rautela A; Biofuel Research Laboratory, School of Biochemical Engineering, IIT(BHU) Varanasi, Varanasi 221005, Uttar Pradesh, India.
  • Yadav I; Biofuel Research Laboratory, School of Biochemical Engineering, IIT(BHU) Varanasi, Varanasi 221005, Uttar Pradesh, India.
  • Gangwar A; Biofuel Research Laboratory, School of Biochemical Engineering, IIT(BHU) Varanasi, Varanasi 221005, Uttar Pradesh, India.
  • Chatterjee R; Biofuel Research Laboratory, School of Biochemical Engineering, IIT(BHU) Varanasi, Varanasi 221005, Uttar Pradesh, India.
  • Kumar S; Biofuel Research Laboratory, School of Biochemical Engineering, IIT(BHU) Varanasi, Varanasi 221005, Uttar Pradesh, India. Electronic address: sanjaykr.bce@iitbhu.ac.in.
Bioresour Technol ; 396: 130432, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38346593
ABSTRACT
Cyanobacteria are the prospective biosolar cell factories to produce a range of bioproducts through CO2 sequestration. Farnesene is a sesquiterpene with an array of applications in biofuels, pest management, cosmetics, flavours and fragrances. This is the first time a codon-optimized farnesene synthase (AFS) gene is engineered into the genomic neutral site of Synechococcus elongatus UTEX 2973 for farnesene synthesis through its endogenous methylerythritol phosphate (MEP) pathway, rendering UTEX AFS strain. Similarly, bottleneck gene(s) of the MEP pathway, 1-deoxy-D-xylulose-5-phosphate synthase (dxs) and/or fusion of isopentenyl diphosphate isomerase and farnesyl diphosphate synthase (idispA) were engineered engendering UTEX AFSdxs, UTEX AFSidispA and UTEX AFSdxsidispA strains. UTEX AFSdxsidispA achieves farnesene productivity of 2.57 mg/L/day, the highest among engineered cyanobacterial strains studied so far. It demonstrates farnesene production, which is 31.3-times higher than the UTEX AFS strain. Moreover, the engineered strains show similar productivity over a three-month period, stipulating the genetic stability of the strains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Synechococcus Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Synechococcus Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido