Your browser doesn't support javascript.
loading
Evaluation of Presence of Enterococcus faecalis in Root Canals of Deciduous Molars with Necrotic Pulp by Agar Culture and Polymerase Chain Reaction.
Nalawade, Triveni M; Bhat, Kishore G; Kale, Alka D; Sogi, Suma; Hugar, Shivayogi M; Kumbar, Vijay M; Mallikarjuna, Rachappa M.
Afiliación
  • Nalawade TM; Department of Paediatric Dentistry, Oman Dental College, Muscat, Oman.
  • Bhat KG; Department of Microbiology, Dr Prabhakar Kore Basic Science Research Center, Belagavi, Karnataka, India.
  • Kale AD; Department of Oral Pathology, KLE Academy of Higher Education and Research (Deemed to be University), V K Institute of Dental Sciences, Belagavi, Karnataka, India.
  • Sogi S; Department of Pedodontics & Preventive Dentistry, M M College of Dental Sciences and Research, Ambala, Haryana, India.
  • Hugar SM; Department of Pedodontics and Preventive Dentistry, KLE Academy of Higher Education and Research (Deemed to be University), V K Institute of Dental Sciences, Belagavi, Karnataka, India.
  • Kumbar VM; Department of Basic Science (Molecular Biology), Dr Prabhakar Kore Basic Science Research Center, Belagavi, Karnataka, India.
  • Mallikarjuna RM; Department of Paediatric Dentistry, Oman Dental College, Muscat, Oman.
Int J Clin Pediatr Dent ; 16(6): 816-819, 2023.
Article en En | MEDLINE | ID: mdl-38344371
ABSTRACT

Objective:

To assess the presence of Enterococcus faecalis in root canals of deciduous molars with necrotic pulp by agar culture and polymerase chain reaction (PCR) assay. Materials and

methods:

This is an experimental study, where a total of 120 endodontic samples were taken from deciduous molars with necrotic pulps. The presence of Enterococcus faecalis was assessed by culture, using Enterococcus confirmatory agar, and by PCR assay. Statistical analysis of the results was performed using McNemar's test.

Results:

The presence of Enterococcus faecalis was detected in 20 samples (16.67% of total) by microbial culture and in 45 samples (37.5% of total) by PCR assay, with a statistically significant difference between the two methods (p < 0.001). Microbial culture and PCR both detect Enterococcus faecalis, with the latter detecting an additional 25 positive samples.

Conclusion:

In this study, PCR assay was significantly more sensitive than agar culture method in detecting the presence of Enterococcus faecalis in root canals of deciduous molars with necrotic pulp, that is, 37.5% of all samples. Clinical

significance:

Importance of presence of Enterococcus faecalis in necrotic pulps of deciduous teeth, as it is primarily responsible for failure of endodontic treatment, thus helping clinicians to advocate the use of local drug delivery in primary teeth endodontics and also aids clinicians in choosing the most effective intracanal medication. How to cite this article Nalawade TM, Bhat KG, Kale AD, et al. Evaluation of Presence of Enterococcus faecalis in Root Canals of Deciduous Molars with Necrotic Pulp by Agar Culture and Polymerase Chain Reaction. Int J Clin Pediatr Dent 2023;16(6)816-819.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Clin Pediatr Dent Año: 2023 Tipo del documento: Article País de afiliación: Omán Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Clin Pediatr Dent Año: 2023 Tipo del documento: Article País de afiliación: Omán Pais de publicación: India