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Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker).
Lin, Dong-Jiang; Zhou, Jiang-Xiong; Ali, Ahmad; Fu, Hua-Ying; Gao, San-Ji; Jin, Lin; Fang, Yong; Wang, Jin-da.
Afiliación
  • Lin DJ; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Zhou JX; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Ali A; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Fu HY; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Gao SJ; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Jin L; College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Fang Y; Hunan Agricultural Biotechnology Research Institute, Hunan Academy of Agriculture science, Changsha, Hunan 410125, PR China.
  • Wang JD; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address: jdwang@fafu.edu.cn.
Int J Biol Macromol ; 262(Pt 2): 129978, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38340916
ABSTRACT
The plant endophytic bacteria are a great source of nature insecticides. However, no such endophytic bacteria have been found in sugarcane, to address this gap, we isolated and identified a strain of Serratia marcescens with moderate insecticidal activity from sugarcane. Taken armyworm Mythimna separata as example, the mortality rates of oral infection and injection infection were 47.06 % and 91 %, respectively. The SM has significant negative affect on the growth, development, and reproduction of M. separata. After determining that these insecticidal substances, 33 potential virulence proteins were screened through the identification and prediction of bacterial proteins. Later we confirmed serralysin was a vital toxic protein from SM that caused M. separata death by prokaryotic expression. In addition, we also found that the intestinal tissue cells infected with SM or serralysin were severely diseased, which may be a major factor in M. separata demise. Finally, through gene expression level, protein molecular docking, we found the aminopeptidase-N would be one of the potential receptors of serralysin. Taken together, our findings indicate that sugarcane endophyte S. marcescens may be beneficial for pest control in sugarcane and explain its insecticidal mechanism. This study provides new ideas and materials for the biological control of pests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Platelmintos / Saccharum / Insecticidas / Mariposas Nocturnas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Platelmintos / Saccharum / Insecticidas / Mariposas Nocturnas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos