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Platelet Proteome Reveals Novel Targets for Hypercoagulation in Pseudoexfoliation Syndrome.
Ugurel, Elif; Narimanfar, Ghazal; Cilek, Neslihan; Kesim, Cem; Altan, Cigdem; Sahin, Afsun; Yalcin, Ozlem.
Afiliación
  • Ugurel E; Research Center for Translational Medicine (KUTTAM), Koc University, Istanbul 34450, Turkey.
  • Narimanfar G; Department of Physiology, School of Medicine, Koc University, Istanbul 34450, Turkey.
  • Cilek N; Research Center for Translational Medicine (KUTTAM), Koc University, Istanbul 34450, Turkey.
  • Kesim C; Research Center for Translational Medicine (KUTTAM), Koc University, Istanbul 34450, Turkey.
  • Altan C; Department of Physiology, School of Medicine, Koc University, Istanbul 34450, Turkey.
  • Sahin A; Department of Ophthalmology, Koc University Medical School, Istanbul 34010, Turkey.
  • Yalcin O; Beyoglu Eye Training and Research Hospital, University of Health Sciences, Istanbul 34421, Turkey.
Int J Mol Sci ; 25(3)2024 Jan 24.
Article en En | MEDLINE | ID: mdl-38338682
ABSTRACT
Pseudoexfoliation syndrome (PEX) is characterized by the accumulation of abnormal extracellular matrix material in ocular and non-ocular tissues, including blood vessel walls. Clot-forming dysfunction might be responsible for venous thrombosis in PEX. We investigated global coagulation, the proteome, and functions of platelets in PEX patients and aimed to determine prognostic biomarkers for thrombosis risk in PEX. Peripheral blood was collected from PEX and retinal vein occlusion (RVO) patients, and age-sex matched controls. Viscoelastic hemostasis was evaluated by rotational thromboelastometry (ROTEM). Platelet markers (CD41, CD42, CD61, and CD62p) and endothelial markers (P-selectin, E-selectin, and von Willebrand factor) were investigated by flow cytometry and ELISA, respectively. The platelet proteome was analyzed by 2D fluorescence difference gel electrophoresis followed by mass spectrometry. Clot formation time (CFT) is significantly reduced in PEX patients compared to the controls (p < 0.05). P-selectin levels were higher in PEX patients than in controls (p < 0.05); E-selectin and von Willebrand factor remained unchanged. The monitorization of CFT by ROTEM, and soluble P-selectin, may help assess thrombotic risk in PEX patients. Proteomic analysis revealed differential expression of Profilin-1 in platelets. Profilin-1 regulates the stability of actin-cytoskeleton and may contribute to impaired platelet hemostatic functions. Increased P-selectin levels together with impaired coagulation dynamics might be responsible for the thrombotic events in PEX disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome de Exfoliación / Trombofilia Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome de Exfoliación / Trombofilia Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Suiza