Hernandulcin Production in Elicited Hairy Roots of Phyla scaberrima: Toward Sustainable Production of a Non-Caloric Sweetener with Nutraceutical Properties.
Chem Biodivers
; 21(3): e202302095, 2024 Mar.
Article
en En
| MEDLINE
| ID: mdl-38334300
ABSTRACT
Herein we report on the generation of hairy root lines of P. scaberrima able to produce hernandulcin (HE), a non-caloric sweetener with nutraceutical properties. From ten different lines analyzed, three synthesized up to 100â
mg â
L-1 HE under the batch culture conditions standardized in this investigation. Adding elicitors (salicylic acid, chitin, Glucanex, polyethylene glycol) and biosynthetic precursors (farnesol and (+)-epi-alpha-bisabolol) significantly altered HE accumulation. Chitin and Glucanex enhanced HE production from 130 to 160â
mg â
L-1 , whereas farnesol and (+)-epi-alpha-bisabolol from 165 to 200â
mg â
L-1 without dependence on biomass accumulation. Improved batch cultures containing liquid Murashige & Skoog medium (MS; pHâ
7), added with 4 % sucrose, 0.5â
mg â
L-1 naphthaleneacetic acid, 100â
mg â
L-1 Glucanex, 150â
mg â
L-1 chitin, 250â
mg â
L-1 farnesol, and 150â
mg â
L-1 (+)-epi-alpha-bisabolol at 25 °C (12â
h light/12â
h darkness), triggered HE accumulation to 250â
mg â
L-1 in 25â
days. The efficiency of each recombinant line is discussed.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sesquiterpenos
/
Edulcorantes
/
Farnesol
/
Sesquiterpenos Monocíclicos
Idioma:
En
Revista:
Chem Biodivers
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Suiza